The pentacyclic triterpenoid hederagenin (1) was subjected to biotransformation by Cunninghamella echinulate CGMCC 3.2000, Mucor subtilissimus CGMCC 3.2454 and Pseudomonas oleovorans CGMCC 1.1641. Three metabolites were obtained. On the basis of nuclear magnetic resonance and high-resolution mass spectral analyses, their structures were characterized as 3β, 23-dihydroxyolean-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester (2), 3β, 15α, 23-trihydroxyolean-12-en-28-oic acid (3), 1β, 3β, 23-trihydroxyolean-12-en-28-oic acid (4), and metabolite (3) was a new compound. This was the first report on the biotransformation of hederagenin.
microbial transformation, Cunninghamella echinulate, Mucor subtilissimus, Pseudomonas oleovorans, hederagenin, triterpenoid
NCBI PubMed ID: 27666872Publication DOI: 10.1080/10286020.2016.1232252Journal NLM ID: 100888334Publisher: Harwood Academic Publishers; London: Informa Healthcare
Correspondence: Feng X
; Chu ZY
Institutions: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China, Navy Medical Research Institute, Shanghai, China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China
Methods: 13C NMR, 1H NMR, TLC, HPLC, extraction, LC-MS, cell growth, HR-ESI-MS, HMBC, NOESY, HSQC
Found