The composition of the polysaccharide moieties of the lipopolysaccharide (LPS) fraction of Burkholderia_Pseudomonas caryophylli is described. Two homopolysaccharide chains, the major one built up of (1→7)-linked a-caryophyllose [3,6,10-trideoxy-4-C-(D-glycero-1-hydroxyethyl)-D-erythro-D-gulo-decose] residues and the minor one made up of (1→7)-linked b-caryose (4,8-cyclo-3,9-di-deoxy-L-erythro-D-ido-nonose) residues, were identified in the LPS fraction. In addition, experimental evidence of a third polysaccharide fraction mainly composed of heptose and glucose is also reported. The structural analysis was performed by chemical and spectroscopic methods.
Pseudomonas caryophylli, Homopolysaccharide, Caryophyllose, Caryose
Publication DOI: 10.1016/0008-6215(96)00019-5Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Dipartimento di Chimica Organica e Biologica, Universita di Napoli Federico II, via Mezzocannone 16, 80134 Naples, Italy, Dipartimento di Science Chimico-Agrarie, Universita di Napoli Federico II, via Universita 100, 80055 Portici, Italy, Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, uiale L. Einaudi 51, 70125 Bari, Italy
Methods: methylation, periodate oxidation, NMR-2D, TLC, acid hydrolysis, GLC, methanolysis, UV, reduction with NaBD4, acetylation
Found