A new N-methoxypyridone analog (1), together with four known compounds, was isolated from the co-culture of Hawaiian endophytic fungi Camporesia sambuci FT1061 and Epicoccum sorghinum FT1062. The structure of the new compound was elucidated as 11S-hydroxy-1-methoxyfusaricide (1) by extensive spectroscopic analysis and comparison with the literature. The absolute configuration of 1 was determined by comparison with the experimental and calculated ECD spectra. The absolute configuration of compound 3 was investigated and renamed as (+)-epipyridone by comparison of the optical rotation and CD spectrum with those of 1. The other known compounds were identified as epicoccarine B (2), D8646-2-6 (4), and iso-D8646-2-6 (5). Compounds 4 and 5 showed modest inhibitory activity towards pathogenic fungi. Epicoccarine B (2) inhibited A2780 and TK-10 with an IC50 value of 22 μM.
co-culture, endophytic fungi, Hawaii, pyridone, tetramic acid
NCBI PubMed ID: 28704977Publication DOI: 10.3390/molecules22071166Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: Li C
; Turkson J ; Sarotti AM ; Yang B ; Cao S
Institutions: Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawai’i at Hilo, Hilo, USA, Cancer Biology Program, University of Hawaii Cancer Center, Honolulu, USA, Instituto de Química Rosario (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina
Methods: 13C NMR, 1H NMR, biological assays, HPLC, extraction, optical rotation measurement, LC-MS, cell growth, HR-ESI-MS
Found