Two new steroids, (24R)-22,23-dihydroxy-ergosta-4,6,8(14)-trien-3-one 23-β-D-glucopyranoside (1), and xylarester (2), together with 16 known compounds were isolated from the extract of endophytic Xylaria sp. solid culture. Their structures were elucidated by spectroscopic methods. Compound 2 has an unprecedent ergosta skeleton with a six-membered lactonic group in A ring. The X-ray single crystal diffraction data of 4,7-dihydroxy-13-tetradeca-2,5,8- trienolide (4) were also reported for the first time. The antibacterial, anti-acetylcholinesterase, nitric oxide inhibition and cytotoxic activities of 1-3 were investigated. Compound 1 showed cytotoxicity to MCF-7 with the ratio of inhibition at 72 % for a concentration at 40 μM.
endophyte, Xylaria sp., steroid
Publication DOI: 10.1177/1934578X1701200617Journal NLM ID: 101477873Publisher: Natural Product Communications
Correspondence: Ding ZT
; Yang YB
Institutions: Yunnan Institute of Microbiology, Yunnan University, Kunming, China, School of Chemical Science and Technology, Yunnan University, Kunming, China, School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, China
Methods: 13C NMR, 1H NMR, IR, HR-ESI-MS, HMBC, COSY
Found