The main component of a water extract of Boletus erythropus fruiting bodies is a Mr 1000000 glucan. The use of classical structural analysis and HMQC (heteronuclear multiple quantum coherence) NMR experiments indicates a (1→3) linked β-D-glucan structure with a single glucose residue attached to O-6 of the main chain and a branching frequency of 1/3.
mushroom, β-D-glucan, Basidiomycetes, Boletus erythropus, water-soluble polysaccharides, HMQC NMR
NCBI PubMed ID: 8862034Publication DOI: 10.1016/0031-9422(96)00234-8Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Laboratoire de Mycologie, Université Paul Sabatier-Toulouse III, Toulouse, France, Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS no. 111, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France
Methods: 13C NMR, 1H NMR, methylation, gel filtration, GC-MS, acid hydrolysis, GC, extraction, acetylation, acetolysis, methylation analysis, reduction, CC, derivatization, HMQC, anthrone-sulfuric acid assay, ion exchange chromatography
Found