We investigated the ability of Trametes versicolor and Pycnoporous cinnabarinus to metabolize triclosan. T. versicolor produced three metabolites, 2-O-(2,4,4'-trichlorodiphenyl ether)-β-D-xylopyranoside, 2-O-(2,4,4'-trichlorodiphenyl ether)-β-D-glucopyranoside, and 2,4-dichlorophenol. P. cinnabarinus converted triclosan to 2,4, 4'-trichloro-2'-methoxydiphenyl ether and the glucoside conjugate known from T. versicolor. The conjugates showed a distinctly lower cytotoxic and microbicidal activity than triclosan did.
glycoside, biotransformation, triclosan, Trametes versicolor, Pycnoporous cinnabarinus
NCBI PubMed ID: 10966448Publication DOI: 10.1128/aem.66.9.4157-4160.2000Journal NLM ID: 7605801Publisher: American Society for Microbiology
Correspondence: kai.hundt@plasmaselect.com
Institutions: Institute of Microbiology and Molecular Biology, Ernst-Moritz-Arndt-University, Greifswald, Germany, Institute of Chemistry and Biochemistry, Ernst-Moritz-Arndt-University, Greifswald, Germany, Südzucker AG Mannheim/Ochsenfurt, ZAFES, Obrigheim, Germany
Methods: 13C NMR, 1H NMR, GC-MS, acid hydrolysis, anion-exchange chromatography, biological assays, HPLC, UV, extraction, CC, cell growth, derivatization
Found