Two hypoglycemic principles, ganoderans B and C, isolated from the fruit bodies of Ganoderma lucidum were shown to be peptidoglycans with Mrs of 7400 and 5800, respectively. Physico-chemical and chemical studies demonstrated that the backbone and side chains of ganoderan B contain D-glucopyranosyl β-1→3 and β-1→6 linkages while those of ganoderan C contain D-glucopyranosyl β-1→3 and β-1→6 linkages and a D-galactopyranosyl α-1→6 linkage.
structure, hypoglycemic activity, Ganoderma lucidum, Polyporaceae, peptidoglycans
Publication DOI: 10.1016/S0031-9422(00)83748-6Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Pharmaceutical Institute, Tohoku University, Sendai, Japan, Kyoritsu College of Pharmacy, Tokyo, Japan
Methods: 13C NMR, 1H NMR, periodate oxidation, SDS-PAGE, acid hydrolysis, GC, Smith degradation, biological assays, enzymatic digestion, extraction, optical rotation measurement, acetylation, methylation analysis, paper electrophoresis, reduction, CC, phenol-sulfuric acid assay, Lowry method