Bisphenol A and its halogenated analogues are commonly used industrial chemicals with strong toxicological effects over many organisms. In this study, metabolic fate of bisphenol A and its halogenated analogues were evaluated with Cunninghamella elegans ATCC36112. Bisphenol A and related analogues were rapidly transformed into several metabolites by C. elegans within 2–4 days. Detailed analysis of metabolites reveals that both phase I and II metabolism occurred in C. elegans. Cytochrome P450-dependent hydroxylation was observed in BPA. However, major reaction with bisphenol A and analogues with 1-2 halogen atoms were the formation of glucose-conjugate, not being inhibited by cytochrome P450 inhibitor. Overall metabolic rates decreased with increasing number of substitution at 2- and 6-position of BPA structures, which may be consequences of limited bioavailability or steric hindrance to conjugate-forming reaction. Information from the current study will provide detailed insights over the fungal metabolism of BPA and analogues.
analogue, glycoside, Cunninghamella elegans, bisphenol A
NCBI PubMed ID: 20455075Publication DOI: 10.1007/s10532-010-9358-8Journal NLM ID: 9100834Publisher: Dordrecht, Boston: Kluwer Academic Publishers
Correspondence: Kim JH
Institutions: Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea, Konkuk University, Seoul, South Korea
Methods: GC-MS, enzymatic digestion, extraction, cell growth, derivatization
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