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Structure type: oligomer
Contained glycoepitopes: IEDB_114701,IEDB_115136,IEDB_140630,IEDB_167188,IEDB_174332

• Article ID: 5336
  Puchart V, Biely P "Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus" - Journal of Biotechnology 137(1-4) (2008) 34-43
  

  When grown on beech-wood glucuronoxylan, two strains of the thermophilic fungus Thermomyces lanuginosius, IMI 84400 and IMI 96213, secreted endo-β-1,4-xylanase of glycoside hydrolase family 11 and simultaneously accumulated an acidic pentasaccharide in the medium. The aldopentaouronic acid was purified and its structure was established by a combination of NMR spectroscopy and enzyme digestion with glycosidases as MeGlcA3Xyl4. Both strains showed limited growth on wheat arabinoxylan as a carbon source. An essential part of the polysaccharide was not utilized, and it was converted to a series of arabinoxylooligosaccharides differing in the degree of polymerization. The structure of the shorter arabinoxylooligosaccharides remaining in the wheat arabinoxylan-spent medium was established using mass spectrometry and digestion with glycosidases. Xylose and linear β-1,4-xylooligosaccharides generated extracellularly during growth on either hardwood or cereal xylan were efficiently taken up by the cells and metabolized intracellularly. The data suggest that due to a lack of extracellular β-xylosidase, α-glucuronidase, and α-l-arabinofuranosidase, the widely used T. lanuginosus strains might become efficient producers of branched xylooligosaccharides from both types of xylans.

  endo-β-1, 4-xylanase, glycoside hydrolase family 11, Thermomyces lanuginosus, aldopentaouronic acid, arabinoxylooligosaccharides

  NCBI PubMed ID: 18680769
  Publication DOI: 10.1016/j.jbiotec.2008.07.1789
  Journal NLM ID: 8411927
  Publisher: Amsterdam: Elsevier
  Correspondence: Biely P
  Institutions: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
  Methods: 13C NMR, 1H NMR, TLC, anion-exchange chromatography, enzymatic digestion, extraction, ESI-QTOF-MS, cell growth
  
   
  
  Aspergillus tubingensis
  CSDB ID 45716 (all data & tools)

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Structure type: oligomer
Trivial name: xylotriose
Contained glycoepitopes: IEDB_114701,IEDB_167188,IEDB_174332

• Article ID: 5336
  Puchart V, Biely P "Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus" - Journal of Biotechnology 137(1-4) (2008) 34-43
  

  When grown on beech-wood glucuronoxylan, two strains of the thermophilic fungus Thermomyces lanuginosius, IMI 84400 and IMI 96213, secreted endo-β-1,4-xylanase of glycoside hydrolase family 11 and simultaneously accumulated an acidic pentasaccharide in the medium. The aldopentaouronic acid was purified and its structure was established by a combination of NMR spectroscopy and enzyme digestion with glycosidases as MeGlcA3Xyl4. Both strains showed limited growth on wheat arabinoxylan as a carbon source. An essential part of the polysaccharide was not utilized, and it was converted to a series of arabinoxylooligosaccharides differing in the degree of polymerization. The structure of the shorter arabinoxylooligosaccharides remaining in the wheat arabinoxylan-spent medium was established using mass spectrometry and digestion with glycosidases. Xylose and linear β-1,4-xylooligosaccharides generated extracellularly during growth on either hardwood or cereal xylan were efficiently taken up by the cells and metabolized intracellularly. The data suggest that due to a lack of extracellular β-xylosidase, α-glucuronidase, and α-l-arabinofuranosidase, the widely used T. lanuginosus strains might become efficient producers of branched xylooligosaccharides from both types of xylans.

  endo-β-1, 4-xylanase, glycoside hydrolase family 11, Thermomyces lanuginosus, aldopentaouronic acid, arabinoxylooligosaccharides

  NCBI PubMed ID: 18680769
  Publication DOI: 10.1016/j.jbiotec.2008.07.1789
  Journal NLM ID: 8411927
  Publisher: Amsterdam: Elsevier
  Correspondence: Biely P
  Institutions: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
  Methods: 13C NMR, 1H NMR, TLC, anion-exchange chromatography, enzymatic digestion, extraction, ESI-QTOF-MS, cell growth
  
   
  
  Aspergillus tubingensis
  CSDB ID 45717 (all data & tools)
• Article ID: 10465
  Kormelink FJM, Gruppen H, Voragen AGJ "Mode of action of (1-4)-β-D-arabinoxylan arabinofuranohydrolase (AXH) and α-L-arabinofuranosidases on alkali-extractable wheat-flour arabinoxylan" - Carbohydrate Research 249 (1993) 345-353
  

  Arabinoxylan-derived oligosaccharides were treated with (1→4)-β-d-arabinoxylan arabinofuranohydrolase (AXH) and two types of α-l-arabinofuranosidase, A and B. Analysis of reaction products by high performance anion-exchange chromatography indicated the removal of arabinofuranosyl groups from singly substituted xylopyranosyl residues. In addition, differences in the specificity of these enzymes towards the various differently substituted oligosaccharides were observed. 1H NMR spectroscopy and methylation analysis of alakli-extractable wheat-flour arabinoxylan treated with AXH confirmed the specificity of AXH towards (1→3)-linked arabinofuranosyl groups on singly substituted xylopyranosyl residues. With these techniques, α-l-arabinofuranosidase B was found to cause minor changes in (1→2)- and (1→3)- linked arabinofuranosyl groups on substituted xylopyranosyl residues.

  NCBI PubMed ID: 8275504
  Publication DOI: 10.1016/0008-6215(93)84099-R
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Department of Food Science, Agricultural University, Biotechnion, Wageningen, The Netherlands
  Methods: 1H NMR, gel filtration, GC-MS, HPAEC, enzymatic digestion, methylation analysis
  
   
  
  Triticum aestivum
  CSDB ID 267431 (all data & tools)
• Article ID: 10466
  Kormelink FJM, Gruppen H, Vietor RJ, Voragen AGJ "Mode of action of the xylan-degrading enzymes from Aspergillus awamori on alkali-extractable cereal arabinoxylans" - Carbohydrate Research 249 (1993) 355-367
  

  Alkali-extractable cereal arabinoxylan and oligosaccharides of known structure derived from it by enzymic hydrolysis were treated with endo-(1→4)-β-d-xylanases I and III from Aspergillus awamori CMI 142717 and the digest subjected to analysis by high performance anion-exchange chromatography. Clear differences in the mode of action of the two endo-(1→4)-β-d-xylanases were observed. When counting from the reducing end, at least one unsubstituted xylopyranosyl residue adjacent to singly substituted xylopyranosyl residues or two unsubstituted xylopyranosyl residues adjacent to doubly substituted xylopyranosyl residues cannot be removed by endo-(1→4)-β-d-xylanase I. At least two unsubstituted xylopyranosyl residues adjacent to singly or doubly substituted xylopyranosyl residues cannot be removed by endo-(1→4)-β-d-Xylosidase from the same xylanolytic system was able to remove terminal xylopyranosyl residues from the nonreducing end of branched oligosaccharides only when two contiguous unsubstituted xylopyranosyl residues were present adjacent to singly or doubly substituted xylopyranosyl residues.

  NCBI PubMed ID: 8275505
  Publication DOI: 10.1016/0008-6215(93)84100-K
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Department of Food Science, Agricultural University, Wageningen, The Netherlands
  Methods: 1H NMR, gel filtration, HPAEC, enzymatic digestion
  
   
  
  Betula, Triticum aestivum
  CSDB ID 117440 (all data & tools)
• Article ID: 10475
  Vietor RJ, Hoffmann RA, Angelino SAGF, Voragen AGJ, Kamerling JP, Vliegenthart JFG "Structures of small oligomers liberated from barley arabinoxylans by endoxylanase from Aspergillus awamori" - Carbohydrate Research 254 (1994) 245-255
  

  Arabinoxylans consisting of more than 90% of arabinose and xylose were extracted from water-in-soluble cell wall material of dehusked barley grain, and degraded with a purified endo-(1→4)-β-d-xylanase from Aspergillus awamori. Twelve of the oligosaccharide fragments released were isolated by a combination of size-exclusion and anion-exchange chromatography, and their structures were determined by 1H NMR spectroscopy. The oligosaccharides identified consisted of (1→4)-linked β-d-xylopyranosyl residues, some of which were substituted at O-2, at O-3, or at both O-2 and O-3, with α-l-arabinofuranosyl groups. Two new structures, a tetrasaccharide and a pentasaccharide, both containing terminal 2-O-arabinofuranosylxylopyranose as a structural element are described.

  NCBI PubMed ID: 8180986
  Publication DOI: 10.1016/0008-6215(94)84257-4
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands, Department of Food Science, Agricultural University, Wageningen, The Netherlands, TNO Biotechnology and Chemistry Institute, Department of Biochemistry and Physical Chemistry, AJ Zeist, The Netherlands
  Methods: 1H NMR, gel filtration, HPAEC, enzymatic digestion
  
   
  
  Hordeum vulgare
  CSDB ID 117667 (all data & tools)