In this paper, microbial transformation of norkurarinone (1) by Cunninghamella blakesleana AS 3.970 was investigated and seven transformed products were isolated and characterized as kurarinone (2), 4″,5″-dihydroxykurarinone (3), 6″-hydroxyl-2'-methoxyl-norkurarinone 7-O-β-D-glucoside (4), 6″-hydroxyl-norkurarinone 4'-O-β-D-glucoside (5), 4″,5″-dihydroxynorkurarinone (6), 7-methoxyl-norkurarinone (7), and 7-methoxyl-4″,5″-dihydroxynorkurarinone (8), respectively. Among them, 3-5 are new compounds, and the glycosylation reaction in microbial transformation process was reported rarely. In addition, the cytotoxicities of transformed products (1-8) were also investigated.
biotransformation, norkurarinone, Cunninghamella blakesleana, lavandulated flavonoid
NCBI PubMed ID: 22924649Publication DOI: 10.1080/10286020.2012.702759Journal NLM ID: 100888334Publisher: Harwood Academic Publishers; London: Informa Healthcare
Correspondence: Yuan QP
; Ma XC
Institutions: School of Pharmacy, Dalian Medical University, Dalian, China, College of Bioengineering, Beijing Polytechnic, Beijing, China, State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China
Methods: 13C NMR, 1H NMR, IR, TLC, UV, extraction, CD, cell growth, melting point determination, FTICR-MS
Found