An acetylated mannan was isolated from the lipopolysaccharide of Pseudomonas diminuta N.C.T.C. 8545 after mild hydrolysis with acid and chromatography of the water-soluble products on Sephadex G-50. Although the presence of a terminal coreoligosaccharide in the mannan could not be demonstrated, chromatographic and electrophoretic studies indicated that the mannan was a real component of the lipopolysaccharide. Degradative and n.m.r. (1H and 13C) studies showed that the mannan had a disaccharide repeating-unit with the following structure, in which about two thirds of the 4-substituted mannopyranosyl residues were acetylated. [formula: see text]
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