Found 421 structures.
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1. Compound ID: 199
Structure type: polymer chemical repeating unit
Trivial name: glucan
Compound class: EPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_232584,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 48
Dueñas-Chasco MT, Rodríguez-Carvajal MA, Tejero-Mateo P, Espartero JL, Irastorza-Iribas A, Gil-Serrano AM "Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77" -
Carbohydrate Research 307(1-2) (1998) 125-133
The exopolysaccharide produced by a ropy strain of Lactobacillus spp. G-77 in a semi-defined medium, was found to be a mixture of two homopolymers composed of D-Glc. The two poly-saccharides were separated and, on the basis of monosaccharide and methylation analyses, 1H, 13C, 1D and 2D NMR experiments, one of the polysaccharides was shown to be a 2-substituted-(1-3)-β-D-glucan, identical to that described for the EPS from Pediococcus damnosus 2.6 (M.T. Duenas-Chasco, M.A. Rodriguez-Carvajal, P. Tejero-Mateo, G. Franco-Rodriguez, J. L. Espartero, A. Irastorza-Iribar, and A.M. Gil-Serrano, Carbohydr. Res., 303 (1997) 453-458), and the other polysaccharide was shown to consist of repeating units with the following structure [formula: see text]
repeating unit, exopolysaccharide, Lactobacillus spp. G-77
NCBI PubMed ID: 9658568Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Departamento de Quimica Aplicada, Facultad de Ciencias Quimicas, Universidad del Pais Vasco, San Sebastian, Spain, Departamento de Quimica Organica, Universidad del Sevillia, Sevillia, Spain, Departamento de Quimica Organica y Farmaceutica, Facultad de Farmacia, Universidad del Sevillia, Sevillia, Spain
Methods: NMR-2D, methylation, NMR, sugar analysis
- Article ID: 3809
Zdorovenko EL, Kachala VV, Sidarenka AS, Izhik AV, Kisileva EP, Shashkov AS, Novik GI, Knirel YA "Structure of the cell-wall polysaccharides of probiotic bifidobacteria Bifidobacterium bifidum BIM B-465" -
Carbohydrate Research 344(17) (2009) 2417-2420
The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacteriumbifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D (1)H-(1)H homonuclear and (1)H-(13)C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure: →6)-α-D-Glcp-(1→3)-β-D-Galf-(1→3)-α-D-Glcp-(1→2)-β-D-Galf-(1→3)-α-D-Galp-(1→3)-α-D-Glcp-(1→ [structure: see the text]. The second isolated polysaccharide is a branched glucan with the main chain of (1→6)-linked α-D-glucopyranose residues, approximately 60% of which are 2-substituted with a single α-D-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.
structure, cell wall polysaccharide, glucogalactan, Bifidobacterium bifidum, probiotic polysaccharide
NCBI PubMed ID: 19804881Publication DOI: 10.1016/j.carres.2009.08.039Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evelina@mail.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GLC-MS, sugar analysis, GLC, NMR-1D
- Article ID: 5832
Pyclik M, Srutkova D, Schwarzer M, Gorska S "Bifidobacteria cell wall-derived exo-polysaccharides, lipoteichoic acids, peptidoglycans, polar lipids and proteins - their chemical structure and biological attributes" -
International Journal of Biological Macromolecules 147 (2020) 333-349
A variety of health benefits has been documented to be associated with the consumption of probiotic bacteria, namely bifidobacteria and lactobacilli. Thanks to the scientific advances in recent years we are beginning to understand the molecular mechanisms by which bacteria in general and probiotic bacteria in particular act as host physiology and immune system modulators. More recently, the focus has shifted from live bacteria towards bacteria-derived defined molecules, so called postbiotics. These molecules may represent safer alternative compared to the live bacteria while retaining the desired effects on the host. The excellent source of effector macromolecules is the bacterial envelope. It contains compounds that are pivotal in the adhesion phenomenon, provide direct bacteria-to-host signaling capacity and the associated physiological impact and immunomodulatory properties of bacteria. Here we comprehensively review the structure and biological role of Bifidobacterium surface and cell wall molecules: exopolysaccharides, cell wall polysaccharides, lipoteichoic acids, polar lipids, peptidoglycans and proteins. We discuss their involvement in direct signaling to the host cells and their described immunomodulatory effects.
exopolysaccharide, Bacterial antigens, Bifidobacterium, peptidoglycan, lipoteichoic acid, probiotics
NCBI PubMed ID: 31899242Publication DOI: 10.1016/j.ijbiomac.2019.12.227Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: schwarzer@biomed.cas.cz; sabina.gorska@hirszfeld.pl
Institutions: Laboratory of Microbiome Immunobiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, Novy Hradek, Czech Republic
- Article ID: 5880
De Vuyst L, De Vin F "Exopolysaccharides from Lactic Acid Bacteria" -
Book: Comprehensive Glycoscience: From Chemistry to Systems Biology. Reference Module in Chemistry, Molecular Sciences and Chemical Engineering (2007) 477-519
carbohydrates, polysaccharides, Lactic acid bacteria, exopolysaccharides, glycolipids, glycoproteins, Glycomics
Publication DOI: 10.1016/B978-044451967-2/00129-XPublisher: Elsevier
Correspondence: ldvuyst@vub.ac.be
Editors: Barchi J, Kamerling H
Institutions: Department of Applied Biological Sciences and Engineering, Research Group of Industrial Microbiology and Food Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium
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2. Compound ID: 276
a-D-Galp-(1-6)-+
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b-D-GlcpNAc-(1-7)-L-gro-a-D-manHepp-(1-6)-a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Glcp-(1--/inner core/ |
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Structure type: oligomer
Aglycon: inner core
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_232584,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 72
Feldman MF, Marolda CL, Monteiro MA, Perry MB, Parodi AJ, Valvano MA "The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat" -
Journal of Biological Chemistry 274(49) (1999) 35129-35138
During O antigen lipopolysaccharide (LPS) synthesis in bacteria, transmembrane migration of undecaprenylpyrophosphate (Und-P-P)-bound O antigen subunits occurs before their polymerization and ligation to the rest of the LPS molecule. Despite the general nature of the translocation process, putative O-antigen translocases display a low level of amino acid sequence similarity. In this work, we investigated whether complete O antigen subunits are required for translocation. We demonstrate that a single sugar, GlcNAc, can be incorporated to LPS of Escherichia coli K-12. This incorporation required the functions of two O antigen synthesis genes, wecA (UDP-GlcNAc:Und-P GlcNAc-1-P transferase) and wzx (O-antigen translocase). Complementation experiments with putative O-antigen translocases from E. coli O7 and Salmonella enterica indicated that translocation of O antigen subunits is independent of the chemical structure of the saccharide moiety. Furthermore, complementation with putative translocases involved in synthesis of exopolysaccharides demonstrated that these proteins could not participate in O antigen assembly. Our data indicate that recognition of a complete Und-P-P-bound O antigen subunit is not required for translocation and suggest a model for O antigen synthesis involving recognition of Und-P-P-linked sugars by a putative complex made of Wzx translocase and other proteins involved in the processing of O antigen
biosynthesis, antigen, structure, O-antigen, O antigen, Escherichia, Escherichia coli, activity, assembly, chemical, chemical structure, putative, sugar
NCBI PubMed ID: 10574995Publication DOI: 10.1074/jbc.274.49.35129Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: mvalvano@julian.uwo.ca
Institutions: Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada, Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
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3. Compound ID: 277
a-D-Glcp-(1-6)-+ a-D-Galp-(1-6)-+
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a-D-Glcp-(1-3)-a-L-Rhap-(1-3)-b-D-GlcpNAc-(1-7)-L-gro-a-D-manHepp-(1-6)-a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Glcp-(1--/inner core/ |
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Structure type: oligomer
Aglycon: inner core
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_158539,IEDB_190606,IEDB_2189047,IEDB_225177,IEDB_232584,IEDB_885823,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 72
Feldman MF, Marolda CL, Monteiro MA, Perry MB, Parodi AJ, Valvano MA "The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat" -
Journal of Biological Chemistry 274(49) (1999) 35129-35138
During O antigen lipopolysaccharide (LPS) synthesis in bacteria, transmembrane migration of undecaprenylpyrophosphate (Und-P-P)-bound O antigen subunits occurs before their polymerization and ligation to the rest of the LPS molecule. Despite the general nature of the translocation process, putative O-antigen translocases display a low level of amino acid sequence similarity. In this work, we investigated whether complete O antigen subunits are required for translocation. We demonstrate that a single sugar, GlcNAc, can be incorporated to LPS of Escherichia coli K-12. This incorporation required the functions of two O antigen synthesis genes, wecA (UDP-GlcNAc:Und-P GlcNAc-1-P transferase) and wzx (O-antigen translocase). Complementation experiments with putative O-antigen translocases from E. coli O7 and Salmonella enterica indicated that translocation of O antigen subunits is independent of the chemical structure of the saccharide moiety. Furthermore, complementation with putative translocases involved in synthesis of exopolysaccharides demonstrated that these proteins could not participate in O antigen assembly. Our data indicate that recognition of a complete Und-P-P-bound O antigen subunit is not required for translocation and suggest a model for O antigen synthesis involving recognition of Und-P-P-linked sugars by a putative complex made of Wzx translocase and other proteins involved in the processing of O antigen
biosynthesis, antigen, structure, O-antigen, O antigen, Escherichia, Escherichia coli, activity, assembly, chemical, chemical structure, putative, sugar
NCBI PubMed ID: 10574995Publication DOI: 10.1074/jbc.274.49.35129Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: mvalvano@julian.uwo.ca
Institutions: Instituto de Investigaciones Bioquimicas Fundacion Campomar, Buenos Aires, Argentina, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada, Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
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4. Compound ID: 305
a-L-Rhap-(1-5)-+
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EtN-(1--P--7)--a-Kdop-(2-4)-+
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a-Kdop-(2-4)-+ |
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L-gro-a-D-manHepp-(1-7)-+ |
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a-D-Galp-(1-6)-+ | P-4)-+ |
| | | |
L-gro-a-D-manHepp-(1-6)-a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/
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P-4)-+ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_133751,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150901,IEDB_151528,IEDB_190606,IEDB_2189047,IEDB_225177,IEDB_226811,IEDB_232584,IEDB_885823,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 81
Frirdich E, Lindner B, Holst O, Whitfield C "Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface" -
Journal of Bacteriology 185(5) (2003) 1659-1671
The waa gene cluster is responsible for the biosynthesis of the lipopolysaccharide (LPS) core region in Escherichia coli and Salmonella: Homologs of the waaZ gene product are encoded by the waa gene clusters of Salmonella enterica and E. coli strains with the K-12 and R2 core types. Overexpression of WaaZ in E. coli and S. enterica led to a modified LPS structure showing core truncations and (where relevant) to a reduction in the amount of O-polysaccharide side chains. Mass spectrometry and nuclear magnetic resonance spectroscopy were used to determine the predominant LPS structures in an E. coli isolate with an R1 core (waaZ is lacking from the type R1 waa gene cluster) with a copy of the waaZ gene added on a plasmid. Novel truncated LPS structures, lacking up to 3 hexoses from the outer core, resulted from WaaZ overexpression. The truncated molecules also contained a KdoIII residue not normally found in the R1 core
Lipopolysaccharide, biosynthesis, LPS, structure, core, gene, isolate, microbiology, strain, polysaccharide, cell, chain, molecule, Research, side chain, Escherichia, Escherichia coli, type, predominant, cluster, gene cluster, O-polysaccharide, O polysaccharide, spectrometry, Salmonella, core region, region, mass spectrometry, reduction, biochemistry, biophysics, hexose, Hexoses, homolog, inner core, lead, Magnetic Resonance Spectroscopy, medicine, modification, modified, nuclear, nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, plasmid, resonance, Salmonella enterica, spectroscopy, surface
NCBI PubMed ID: 12591884Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: cwhitfie@uoguelph.ca
Institutions: Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Biophysics. Analytical Biochemistry, Research Center Borstel, Center for Medicine and Biosciences, D-23845 Borstel, Germany
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5. Compound ID: 307
a-L-Rhap-(1-5)-+
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EtN-(1--P--7)--a-Kdop-(2-4)-+
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a-Kdop-(2-4)-+ |
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EtN-(1---P---P---4)-+ |
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a-D-Galp-(1-6)-+ | P-4)-+ |
| | | |
Hepp-(1-6)-a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/
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L-gro-a-D-manHepp-(1-7)-+ |
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Structure type: oligomer
Aglycon: lipid A
Trivial name: core oligosaccharide
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130659,IEDB_130670,IEDB_133751,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_150901,IEDB_151528,IEDB_190606,IEDB_2189047,IEDB_225177,IEDB_226811,IEDB_232584,IEDB_885823,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 81
Frirdich E, Lindner B, Holst O, Whitfield C "Overexpression of the waaZ gene leads to modification of the structure of the inner core region of Escherichia coli lipopolysaccharide, truncation of the outer core, and reduction of the amount of O polysaccharide on the cell surface" -
Journal of Bacteriology 185(5) (2003) 1659-1671
The waa gene cluster is responsible for the biosynthesis of the lipopolysaccharide (LPS) core region in Escherichia coli and Salmonella: Homologs of the waaZ gene product are encoded by the waa gene clusters of Salmonella enterica and E. coli strains with the K-12 and R2 core types. Overexpression of WaaZ in E. coli and S. enterica led to a modified LPS structure showing core truncations and (where relevant) to a reduction in the amount of O-polysaccharide side chains. Mass spectrometry and nuclear magnetic resonance spectroscopy were used to determine the predominant LPS structures in an E. coli isolate with an R1 core (waaZ is lacking from the type R1 waa gene cluster) with a copy of the waaZ gene added on a plasmid. Novel truncated LPS structures, lacking up to 3 hexoses from the outer core, resulted from WaaZ overexpression. The truncated molecules also contained a KdoIII residue not normally found in the R1 core
Lipopolysaccharide, biosynthesis, LPS, structure, core, gene, isolate, microbiology, strain, polysaccharide, cell, chain, molecule, Research, side chain, Escherichia, Escherichia coli, type, predominant, cluster, gene cluster, O-polysaccharide, O polysaccharide, spectrometry, Salmonella, core region, region, mass spectrometry, reduction, biochemistry, biophysics, hexose, Hexoses, homolog, inner core, lead, Magnetic Resonance Spectroscopy, medicine, modification, modified, nuclear, nuclear magnetic resonance, nuclear magnetic resonance spectroscopy, plasmid, resonance, Salmonella enterica, spectroscopy, surface
NCBI PubMed ID: 12591884Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: cwhitfie@uoguelph.ca
Institutions: Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Biophysics. Analytical Biochemistry, Research Center Borstel, Center for Medicine and Biosciences, D-23845 Borstel, Germany
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6. Compound ID: 695
b-L-Arap4N-(1--P--6)--a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
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a-D-Glcp-(1-3)-a-D-Manp-(1-6)-+ |
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a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
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a-Kdop-(2-3)-+ |
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Structure type: oligomer
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
- Article ID: 517
Vinogradov E, Li J, Sadovskaya I, Jabbouri S, Helander IM "The structure of the carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-79104" -
Carbohydrate Research 339(9) (2004) 1637-1642
The structure of the carbohydrate backbone of the lipopolysaccharide from Pectinatus frisingensis strain VTT E-79104 was analyzed using chemical degradations, NMR spectroscopy, mass spectrometry, and chemical methods. The LPS contains two major structural variants, differing in the presence or absence of an octasaccharide fragment. The largest structure of the carbohydrate backbone of the LPS, that could be deduced from experimental results, consists of 20 monosaccharides arranged in a nonrepetitive sequence: where R is H or 4-O-Me-α-L-Fuc-(1-2)-4-O-Me-β-Hep-(1-3)-α-GlcNAc-(1-2)-β-Man -(1-3)-β-ManNAc-(1-4)-α-Gal-(1-4)-β-Hep-(1-3)-β-GalNAc-(1- where Hep is a residue of D-glycero-D-galacto-heptose; all monosaccharides have the D-configuration except for 4-O-Me-L-Fuc and L-Ara4N. This structure is architecturally similar to the oligosaccharide system reported previously in P. frisingensis VTT E-82164 LPS, but differs from the latter in composition and also in the size of the outer region.
Lipopolysaccharide, core, lipid A, Pectinatus frisingensis
NCBI PubMed ID: 15183738Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa ON, Canada K1A 0R6, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite du Littoral-Cote d'Opale, Bassin Napoleon BP 120, F-62327, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, FIN-00014, Helsinki, Finland
Methods: NMR-2D, methylation, NMR, sugar analysis, MS
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7. Compound ID: 696
b-L-Arap4N-(1--P--6)--a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
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b-D-GlcpNAc-(1-4)-+ a-D-6dTalp2Ac3Me-(1-3)-+ a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-+ |
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a-D-GlcpNAc-(1-3)-a-D-Fucp-(1-2)-b-D-GlcpA-(1-4)-a-D-Fucp-(1-3)-a-D-GalpNAc-(1-4)-b-D-ManpNAc-(1-4)-a-D-Galp-(1-3)-b-D-GalpNAc-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
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a-Kdop-(2-3)-+ |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_130648,IEDB_130650,IEDB_130659,IEDB_130701,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_140116,IEDB_140630,IEDB_141584,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144990,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_167071,IEDB_190606,IEDB_232584,IEDB_423153,IEDB_885813,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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8. Compound ID: 697
a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
|
a-D-Glcp-(1-3)-a-D-Manp-(1-6)-+ |
| |
a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
a-Kdop-(2-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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9. Compound ID: 699
a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
|
b-D-GlcpN-(1-4)-+ a-D-6dTalp3Me-(1-3)-+ a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-+ |
| | | |
a-D-GlcpN-(1-3)-a-D-Fucp-(1-2)-b-D-GlcpA-(1-4)-a-D-Fucp-(1-3)-a-D-GalpN-(1-4)-b-D-ManpN-(1-4)-a-D-Galp-(1-3)-b-D-GalpN-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
a-Kdop-(2-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140116,IEDB_140630,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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10. Compound ID: 700
a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
|
b-D-GlcpN-(1-4)-+ a-D-6dTalp3Me-(1-3)-+ a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-+ |
| | | |
a-D-GlcpN-(1-3)-a-D-Fucp-(1-2)-a-D-GlcpA-(1-4)-a-D-Fucp-(1-3)-a-D-GalpN-(1-4)-b-D-ManpN-(1-4)-a-D-Galp-(1-3)-b-D-GalpN-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
a-Kdop-(2-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140116,IEDB_140630,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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11. Compound ID: 701
a-D-Glcp-(1-3)-a-D-Manp-(1-6)-+ a-Kdop-(2-4)-+
| |
a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-D-2,5anhMan-ol
|
a-Kdop-(2-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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12. Compound ID: 703
a-D-Glcp-(1-3)-a-D-Manp-(1-6)-+
|
a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo-ol |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136906,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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13. Compound ID: 704
b-D-GlcpNAc-(1-4)-+ a-D-6dTalp3Me-(1-3)-+ a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-+
| | |
a-D-GlcpNAc-(1-3)-a-D-Fucp-(1-2)-b-D-GlcpA-(1-4)-a-D-Fucp-(1-3)-a-D-GalpNAc-(1-4)-b-D-ManpNAc-(1-4)-a-D-Galp-(1-3)-b-D-GalpNAc-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo-ol |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_130648,IEDB_130701,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_140116,IEDB_140630,IEDB_141584,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144990,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_167071,IEDB_190606,IEDB_232584,IEDB_423153,IEDB_885813,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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14. Compound ID: 705
b-D-GlcpNAc-(1-4)-+ a-D-6dTalp2Ac3Me-(1-3)-+ a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-+
| | |
a-D-GlcpNAc-(1-3)-a-D-Fucp-(1-2)-b-D-GlcpA-(1-4)-a-D-Fucp-(1-3)-a-D-GalpNAc-(1-4)-b-D-ManpNAc-(1-4)-a-D-Galp-(1-3)-b-D-GalpNAc-(1-3)-a-D-Glcp-(1-3)-a-D-Manp-(1-6)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-Kdo |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_115015,IEDB_115136,IEDB_130648,IEDB_130650,IEDB_130701,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_140116,IEDB_140630,IEDB_141584,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_144990,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_167071,IEDB_190606,IEDB_232584,IEDB_423153,IEDB_885813,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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15. Compound ID: 707
a-D-GlcpN-(1-4)-a-Kdop-(2-4)-+
|
a-D-Glcp-(1-2)-a-D-Manp-(1-6)-+ |
| |
a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-b-D-GlcpN-(1-6)-a-D-GlcpN-(1-P
|
a-Kdop-(2-3)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 186
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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Next 15 structure(s)
Total list of structure IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (record IDs):
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