The chemical structure of a water insoluble α-glucan isolated from the cell wall of Aspergillus wentii was described on the basis of total acid hydrolysis, methylation analysis, and 1D and 2D NMR studies (TOCSY, DQF-COSY, NOESY and HSQC) as well as other instrumental techniques. It was established that the analyzed preparation contained a linear polymer composed almost exclusively of (1→3)-linked α-D-glucose, with a molecular mass of about 850kDa. The polymer was divided into subunits separated by a short spacers of (1→4)-linked α-D-glucoses. Each subunit contained about 200 glucose residues.
NMR, FT-IR, (1-3)-α-D-Glucan, water-insoluble polysaccharide, Aspergillus wentii
NCBI PubMed ID: 23121952Publication DOI: 10.1016/j.carbpol.2012.08.060Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: adam.choma@poczta.umcs.lublin.pl
Institutions: Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland, Department of Genetics and Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Lublin, Poland
Methods: 1H NMR, methylation, GC-MS, sugar analysis, acid hydrolysis, Smith degradation, FTIR, GPC, optical rotation measurement, acetylation, TOCSY, NaBH4 reduction, DQF-COSY, NOESY, HSQC, immunofluorescent labelling, β-elimination
Found