A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.
Lipopolysaccharide, NMR, antigen, structure, blotting, chemistry, Bacterial, human, DNA, strain, structural, polysaccharide, O-antigen, analysis, O antigen, group, O-antigenic, O-antigenic polysaccharide, acid, Acinetobacter, antibodies, antibody, epitope, monoclonal, monoclonal antibodies, monoclonal antibody, animal, antigens, Carbohydrate Sequence, immunization, immunology, mice, Inbred BALB C, Molecular Sequence Data, epitopes, NMR spectroscopy, Cross Reactions, O antigens, O-antigens, bacteria, hydrolysis, serological, biological, Magnetic Resonance Spectroscopy, spectroscopy, gel permeation chromatography, Western, Carbohydrate Conformation, chromatography, classification, fucose, serotyping
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