The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.
sophorolipids, arachidonic acid, 20-hydroxyeicosatetraenoic acid
NCBI PubMed ID: 23966667Publication DOI: 10.1194/jlr.D042226Journal NLM ID: 0376606Publisher: ASBMB
Correspondence: Hammock BD
; Zhang G
Institutions: Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium, Department of Entomology and Comprehensive Cancer Center, University of California-Davis, Davis, USA, Department of Food Science, University of Massachusetts-Amherst, Amherst, USA, Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University, Nanjing, China
Methods: HPLC, extraction, LC-MS
Found