The exocellular polysaccharide produced by the cyanobacterium Cyanospira capsulata has been subjected to partial acid hydrolysis and N-deacetylation-nitrous acid deamination. The oligosaccharides released have been isolated by weak anion exchange and aqueous size exclusion chromatography, and characterized by a combination of 1D and 2D nuclear magnetic resonance spectroscopy, mass spectrometry, sugar composition and linkage analyses. The polysaccharide has an octasaccharide repeating unit with the following structure: [formula: see text]
structure, exopolysaccharide, Cyanospira capsulata, 4-O-(1-carboxyethyl)mannose, blue-green algae, cyanobacterium
NCBI PubMed ID: 9658567Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: gimpa@dipchi.unict.it
Institutions: Istituto per la Chimica e la Tecnologia dei Materiali Polimerici, Consiglio Nazionale delle Ricerche, Catania, Italy, Dipartimento di Scienze Chimiche, Universita di Catania, Viale A. Doria 6, 95125 Catania, Italy
Methods: NMR-2D, partial acid hydrolysis, NMR, MS, de-N-acetylation/deamination, HPAEC-PAD