Sophorolipids (SLs), a prominent member of the biosurfactants family are produced in acidic and/or lactonic form by yeast Starmerella bombicola NRRL Y-17069 when grown on hydrophilic or hydrophobic or both carbon sources. In current study, ricinoleic acid rich castor oil (10%) was used as hydrophobic and glycerol (10%) was used as hydrophilic carbon source. The yields of 24.5 ± 0.25 g/l sophorolipids were analyzed by anthrone and HPLC method which further increased upto 40.24 ± 0.76 g/l sophorolipids using fed batch process at 5L fermenter level. The structures of sophorolipids synthesized on castor oil were elucidated by liquid chromatography-mass spectrometer (LC-MS), (13)C and (1)H NMR. The results indicated that the ricinoleic acid (RA) gets hydroxylated at ω-1 position but incorporated into sophorolipids through already available hydroxyl group at 12(th) position. It resulted in the production of a novel sophorolipids with hydroxyl fatty acid as side chain and has applications as surfactant for novel drug delivery, anti microbial agent, cosmetic ingredient and emulsifier.
sophorolipids, ricinoleic acid, castor oil, Starmerella bombicola (NRRL Y-17069), direct incorporation, ω-1 hydroxylation
NCBI PubMed ID: 25757436Publication DOI: 10.5650/jos.ess14214Journal NLM ID: 101175339Publisher: Tokyo: Japan Oil Chemists Society
Correspondence: udayannapure@gmail.com
Institutions: Food Engineering and Technology Department, Institute of Chemical Technology, Mumbai, India
Methods: 13C NMR, 1H NMR, TLC, GC, FTIR, HPLC, extraction, LC-MS, centrifugation, anthrone method, gravimetric measurement
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