The (1→6)-β-D-glucans from Agaricus bisporus and Agaricus brasiliensis were purified to evaluate their effects on the innate immune system. THP-1 macrophages were used to investigate the induction of the expression of TNF-α, IL1β, and COX-2 by RT-PCR. The purification of the polysaccharides gave rise to fractions containing 96-98% of glucose. The samples were analyzed by GC-MS, HPSEC and (13)C NMR, which confirmed the presence of homogeneous (1→6)-β-D-glucans. The β-glucans were incubated with THP-1 derived macrophages, for 3 h and 6 h to evaluate their effects on the expression of pro-inflammatory genes. Both β-glucans stimulated the expression of such genes as much as the pro-inflammatory control (LPS). When the cells were incubated with LPS+β-glucan, a significant inhibition of the expression of IL-1β and COX-2 was observed for both treatments after 3 h of incubation. By the results, we conclude that the (1→6)-β-D-glucans present an immunostimulatory activity when administered to THP-1 derived macrophages.
Immunostimulatory activity, Agaricus bisporus, (1→6)-β-D-glucan, Agaricus brasiliensis
NCBI PubMed ID: 23544515Publication DOI: 10.1016/j.carbpol.2012.12.073Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Van Griensven LJ
; Van Griensven LJ
Institutions: Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Wageningen University and Research Centre, Wageningen, The Netherlands, Plant Research International, Wageningen University and Research, Wageningen, The Netherlands
Methods: 13C NMR, methylation, GC-MS, HPSEC, extraction, acetylation, NaBH4 reduction, dialysis, centrifugation, TFA hydrolysis, precipitaion, cytokine assays
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