Ethyl acetate extracts of Armillaria tabescens (strain JNB-OZ344) showed significant fungistatic and bacteristatic activities against several major human pathogens including Candida albicans, Cryptococcus neoformans, Escherichia coli and Mycobacterium intracellulare. Chemical analysis of these extracts led to the isolation and identification of four new compounds, emestrin-F (1), emestrin-G (2), 6-O-(4-O-methyl-β-D-glucopyranosyl)-8-hydroxy-2,7-dimethyl-4H-benzopyran-4-one (3) and cephalosporolide-J (4), along with five other previously known compounds, emestrin (5), cephalosporolide-E (6), decarestrictine-C2 (7), ergosterol and brassicasterol. Structural elucidation of all compounds was carried out by NMR and MS analyses. Antimicrobial assays revealed that compounds 1 and 5 were responsible for the observed growth inhibitory activities of the fungal extracts against the human pathogens tested.
antimicrobial activity, Armillaria tabescens, emestrin, chromone, cephalosporolide
NCBI PubMed ID: 23140424Publication DOI: 10.1080/14786419.2012.738206Journal NLM ID: 101167924Publisher: Milton Park, UK : Taylor & Francis Health Sciences
Correspondence: Nanayakkara NP
Institutions: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, MS, USA, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, MS, USA, Center for Bottomland Hardwoods Research, USDA-Forest Service, Southern Hardwood Laboratory, Stoneville, MS, USA, Crop Genetics and Production Research Unit, USDA-ARS, Stoneville, MS, USA
Methods: 13C NMR, 1H NMR, TLC, HR-ESI-MS, HMBC, HMQC, COSY
Found