Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 16313893Publication DOI: 10.1016/j.carres.2005.11.001Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Peng G. Wang <wang.892
osu.edu>
Institutions: Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA, Department of Chemistry, University of Maryland, Baltimore County, MD 21250, USA
The structure of a new O-polysaccharide from Escherichia coli O86:K62:B7 was determined using NMR and methylation analysis. The structure is as follows: [carbohydrate: see text]. Comparison with the previously published structure from E. coli O86:K2:H2 revealed that the O-polysaccharides from these two E. coli O86 serotypes share the same branched pentasaccharide repeating unit. However, they differ in the anomeric configuration of the linkage, the linkage position, and the identity of the residue through which polymerization occurs. The immunochemical activity of these two forms of LPS toward anti-B antibody was studied and compared. The results showed that LPS from E. coli O86:K2:H2 strain possesses higher blood group B reactivity. The immunoreactivity difference was explained by modeling of the O-repeating unit tetrasaccharide fragments. This finding provides a good system for the further study of O-polysaccharide biosynthesis especially the repeating unit polymerization mechanism.
Lipopolysaccharide, O-polysaccharide, molecular modeling, blood group antigens, polymerization, O-Repeating unit, Blood group antigens; Lipopolysaccharide; Molecular modeling; O-Polysaccharide; O-Repeating unit; Polymerization
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract, p.101, fig 1 B
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_140125,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_142489,IEDB_143260,IEDB_144562,IEDB_149558,IEDB_150766,IEDB_150948,IEDB_150952,IEDB_151528,IEDB_152212,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_241096,IEDB_461710,IEDB_461718,IEDB_461719,IEDB_549285,IEDB_885822,IEDB_918314,SB_148,SB_154,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_86,SB_87,SB_88
Methods: methylation, GC-MS, NMR, ELISA, composition analysis
Biological activity: serological data, biological activities data
Enzymes that release or process the structure: polymerase wzy
3D data: molecular modeling
Related record ID(s): 10715, 11314, 11375, 20226, 20508, 20618, 21563, 22686, 30311
NCBI Taxonomy refs (TaxIDs): 2162909Reference(s) to other database(s): GTC:G64231CD, GlycomeDB:
27794
Show glycosyltransferases
There is only one chemically distinct structure:
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