Sophorolipids (SLs) were synthesized by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 grown on fish oil and glucose. They were purified using preparative HPLC and their structures were identified by MS/MS. The yields of total and lactonic SLs were 47 and 19 g l(-1) in shake-flasks when fish oil 4 % (v/v) was used with glucose in the medium. The composition of SL mixture contained more than 20 SL molecules. Several unconventional SL molecules with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) including zero-, mono- and di-acetylated acidic SLs with EPA and a di-acetylated acidic SL with DHA were obtained. Two unconventional lactonic SL molecules, non-acetylated lactonic SL with 22:3 and non-acetylated lactonic SL with 20:0, were also obtained.
sophorolipids, Wickerhamiella domercqiae, docosahexaenoic acid, eicosapentaenoic acid, fish oil
NCBI PubMed ID: 23386226Publication DOI: 10.1007/s10529-013-1151-4Journal NLM ID: 8008051Publisher: Kluwer Academic Publishers
Correspondence: Ma XJ
; Wang S ; Song X
Institutions: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China, State Key Laboratory of Microbial Technology and National Glycoengineering Research Centre, Shandong University, Jinan, Shandong, China
Methods: GC-MS, ESI-MS, HPLC, CID-MS
Found