An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 10(4) Da, which was named ABP, and its main components were D-glucose and D-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-D-Glcp as the main backbone with (1→4)-linked α-D-Manp units. The structure of the mainly repeating units of ABP was established as: →6)-β-D-Glcp-(1→4)-α-D-Manp(1→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→.
polysaccharide, Structural characterization, NMR spectrum, Agaricus bisporus
NCBI PubMed ID: 24406732Publication DOI: 10.3390/ijms15010787Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: He J
; Zhang A ; Ru Q ; Dong D ; Sun P
Institutions: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China, Yuhang District Agricultural Monitoring Centre of Hangzhou, Hangzhou, China
Methods: 13C NMR, 1H NMR, IR, GC-MS, HPLC, GPC, HPSEC, acetylation, TOCSY, methylation analysis, reduction with NaBH4, GC-FID, HMBC, HMQC, COSY, NOESY, FT-IR, TFA hydrolysis
Found