CSDB: Search results

References to other databases: GTC:G56050EN, GlycomeDB:25195
Structural formula & atomic coordinates
Sweet-II 3D model
Show glycosyltransferases

The structure of the extracellular polysaccharide (EPS) produced by the Rhizobium sp. B strain isolated from atypical nodules on alfalfa has been determined using a combination of chemical and physical techniques (methylation analysis, high pH-anion exchange chromatography (HPAEC), mass spectrometry and 1-D and 2-D NMR spectroscopy). As opposed to the EPS from other strains of Rhizobium, the EPS from the sp. B strain contains D-Glc together with L-Rha and 2-deoxy-D-arabino-hexuronic acid. It is a polymer of a repeating unit having the following structure: →4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-β-D-Glcp-(1→4)-2-deoxy-β-D-GlcpA-(1→. The polysaccharide also contains 0.6 O-acetyl groups per sugar which have not been located.

structure, polysaccharide, acid, Rhizobia, Rhizobium, structural analysis, extracellular polysaccharide, species, 2-deoxy-arabino-D-hexuronic acid, Rhizobium sp.

NCBI PubMed ID: 11434374
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: josiane.courtois@iut.u-picardie.fr
Institutions: Laboratoire des Polysaccharides Microbiens et Vegetaux, Departement de Genie Biologique, Institut Universitaire de Technologie, Universite de Picardie Jules Verne, Avenue des Facultes, F-80025 Amiens, France, Centre de Recherches sur les Macromolecules Vegetales, UPR CNRS 5301, BP 53 F- 38041, Grenoble, France
Methods: methylation, NMR-2D, partial acid hydrolysis, NMR, carboxyl reduction, MS

The exocellular polysaccharide S-7, a heteropolysaccharide from Azotobacter indicus variant myxogenes has been studied using methylation analysis, Smith degradation, partial acid hydrolysis, NMR spectroscopy and mass spectrometry as the principal methods. It is concluded that the repeating unit has the following structure: [structure: see text] The absolute configuration of the deoxyhexuronic acid was deduced from 1H NMR chemical shifts and is most likely D. Approximately two O-acetyl groups per repeating unit are present, one of which is presumably on the Rha residue. The structure bears great resemblance to another polysaccharide, recently studied, produced by Sphingomonas paucimobilis I-886.

structure, capsular polysaccharide, deoxyhexuronic acid, Azotobacter indicus

NCBI PubMed ID: 11383898
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: pererik.jansson@kfcmail.hs.sll.se
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden, University College of South Stockholm, S-141 04 Huddinge, Sweden, Clinical Research Center, Analytical Unit, Karolinska Institute, Huddinge Hospital, Novum, S-141 86 Huddinge, Sweden
Methods: methylation, partial acid hydrolysis, NMR, Smith degradation

Bacterial extracellular polysaccharides are known as a cell-bound capsule, a sheath, or a slime, which is excreted into the environment. They play an important role in virulence of medical bacteria and plant-to-symbiont interaction and are used for serotyping of bacteria and production of vaccines. Some exopolysaccharides have commercial applications in industry, and claims of health benefits have been documented for an increasing number of them. Exopolysaccharides have diverse composition and structure, and some contain sugar and non-sugar components that are found in bacterial carbohydrates only. The present article provides an updated collection of the data on exopolysaccharides of various classes of gram-negative and gram-positive bacteria reported until the end of 2019. When known, biosynthesis pathways of exopolysaccharides are treated in a summary manner. References are made to structure and biosynthesis relatedness between exopolysaccharides of different bacterial taxa as well as between bacterial polysaccharides and mammalian glycosaminoglycans.

polysaccharide structure, Gram-negative bacteria, capsule, Biofilm, polysaccharide biosynthesis, gram-positive bacteria, Monosaccharide composition, Bacterial exopolysaccharide, non-sugar component

Publication DOI: 10.1016/B978-0-12-819475-1.00005-5
Publisher: Elsevier
Correspondence: marie-rose.vancalsteren@canada.ca; yknirel@gmail.com
Editors: Barchi J, Kamerling H
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, QC, Canada

structure, capsular polysaccharide, deoxyhexuronic acid, Azotobacter indicus