Structural studies on cerebroside isolated from Aspergillus oryzae were carried out using mainly gas-liquid chromatography-mass spectrometry. The major component fatty acids were 2-hydroxystearic and 2-hydroxy-trans-octadecenoic acid; branched 17?-methyl nonadecasphingadienine isomers were the predominant long-chain bases. The component sugar was only glucose. The structure of the major cerebrosides was assumed to be N-2'-hydroxystearoyl-1-O-glucosyl-17?-methyl sphingadienine and N-2'-hydroxyoctadecenoyl-1-O-glucosyl-17?-methyl sphingadienine.
cerebroside
NCBI PubMed ID: 1009131Publication DOI: 10.1016/0005-2760(77)90080-7Journal NLM ID: 0217513Publisher: Elsevier
Institutions: Department of Agricultural Chemistry, Obihiro University, Obihiro, Japan
Methods: 1H NMR, GLC-MS, IR, TLC, acid hydrolysis, GLC, methanolysis, extraction, CC, alcaline hydrolysis