Five new steroidal saponins (1-5) were isolated from the fermentation broth of total furostanol glycosides from tubers of Dioscorea zingiberensis C.H. Wright incubated with a fungal, Absidia coerulea AS 3.3389, along with known saponins, zingiberensis new saponin (6), deltonin (7), prosapogenin A of dioscin (8), and protobioside (9), and their structures were established by NMR spectroscopy and mass spectrometry as well as by comparison with previously reported spectral data in the literatures. The induced effects in vitro on rat platelet aggregation of all compounds were evaluated
biotransformation, Absidia coerulea, Dioscorea zingiberensis, Dioscoreaceae, steroidal saponins, induced-platelet aggregation activity
NCBI PubMed ID: 25498025Publication DOI: 10.1016/j.carres.2014.11.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Ma BP
Institutions: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China, Center for Culture Collection of Pharmaceutical Microorganisms, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China, College of Pharmacy, Inner Mongolia Medical University, Hohhot, China
Methods: 13C NMR, 1H NMR, TLC, biological assays, HPLC, extraction, optical rotation measurement, CC, cell growth, HR-ESI-MS, centrifugation
Found