The structure of a capsular polysaccharide (CPS) from a clinical isolate of Bacteroides vulgatus was elucidated. B. vulgatus IMCJ 1204 was isolated from feces of a patient with Crohn's disease. CPS was prepared by phenol/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. The structure of CPS was determined by chemical analysis and NMR spectroscopy including DQF-COSY, TOCSY, ROESY, HSQC-TOCSY, HMQC and HMBC to be a polysaccharide composed of the following repeating unit: →3)β-D-Glcp(1→6)[α-D-GalpNAc(1→2)β-D-Galp(1→4)]β-D-GlcpNAc(1→3)α-D-Galp(1→4)β-D-Manp(1→.
NMR, structure, clinical, disease, isolate, structural, capsular, polysaccharide, capsular polysaccharide, elucidation, Bacteroides, Bacteroides vulgatus
NCBI PubMed ID: 11389714Publication DOI: 10.1046/j.1432-1327.2001.02147.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: tkirikae@ri.imcj.go.jp
Institutions: Research Institute, International Medical Center of Japan, Tokyo, Japan, Graduate School of Science, Osaka University, Japan
Methods: NMR-2D, NMR, chemical analysis
Found