A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained β-L-arabinose, 6-deoxy-β-D-altro-heptose (β-D-6d-altHep) and 6-deoxy-β-L-altrose (β-L-6d-Alt), whereas in the minor trisaccharide the β-L-6d-Alt is replaced by its C-5 epimer α-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established: [formula: see text]
Campylobacter jejuni, L-Arabinose, O41 polysaccharide structure, 6-deoxy-D-altro-heptose, 6-Deoxy-L-altrose, D-fucose
NCBI PubMed ID: 10520260Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: angela.savage@nuigalway.ie
Institutions: Department of Microbiology, National University of Ireland, Galway, Ireland, Department of Chemistry, National University of Ireland, Galway, Ireland, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow R- 17913, Russia
Methods: NMR-2D, NMR, acid hydrolysis
Found