A comprehensive characterization of polysaccharides from the chanterelle was performed. Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC. A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography. The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-D-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-D-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-D-glucan derived from Saccharomyces cerevisiae which was used as reference.
NMR, mannan, glucan, Saccharomyces cerevisiae, Basidiomycota, Chanterelle
NCBI PubMed ID: 27112866Publication DOI: 10.1016/j.carbpol.2016.03.052Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Nyman AA
; Aachmann FL ; Rise F ; Ballance S ; Samuelsen AB
Institutions: School of Pharmacy, University of Oslo, Blindern, Oslo, Norway, Department of Biotechnology, NOBIPOL, Norwegian University of Science and Technology, Trondheim, Norway, Department of Chemistry, University of Oslo, Blindern, Oslo, Norway, Nofima, Norwegian Institute of Food, Fisheries and Aquaculture Research, Aas, Norway
Methods: 13C NMR, 1H NMR, periodate oxidation, GC-MS, GC, Smith degradation, linkage analysis, extraction, reduction with NaBD4, acetylation, TOCSY, methylation analysis, SEC, SEC-MALLS, HMBC, COSY, NOESY, HSQC, TFA hydrolysis, HCl methanolysis
Found