An acidic O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudoalteromonas haloplanktis ATCC14393 and found to contain D-galactose, 3-(N-acetyl-D-alanyl)amino-3,6-dideoxy-D-glucose (DQui3NxDAlaAc), 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-QuiNAc4NAc), 2-acetamido-2-deoxy-D- and -L-galacturonic acids (D- and L-GalNAcA), and O-acetyl groups. On the basis of Smith degradation and 1H and 13C NMR spectroscopic studies, including 2D COSY, TOCSY, NOESY, 1H, 13C HMQC, and HMBC experiments, the following structure of the pentasaccharide repeating unit of the polysaccharide was established: -4)-a-L-GalpNAcA-(1-3)-b-D-QuipNAc4NAc-(1-2)-b-D-Quip3NxDAlaAc-(1-4)-a-D-GalpNAcA-(1-4)-a-D-Galp2,6Ac2-(1- where O-acetylation of the galactose residue at each position is partial (50-70%).
NMR spectroscopy, O-Specific polysaccharide structure, 2, 4, Pseudoalteromonas haloplanktis, L-iduronic acid, 4-diamino-2, 6-trideoxy-D-glucose, (S)-3-hydroxybutyric acid
Publication DOI: 10.1016/S0008-6215(97)10108-2Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: angela.savage@nuigalway.ie
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Department of Chemistry, National University of Ireland, Galway, Ireland, Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russian Federation
Methods: NMR-2D, NMR, Smith degradation
Found