Show legend Show as text

Structure type: monomer
Trivial name: riboflavin, vitamin B2
Compound class: glycoside
Contained glycoepitopes: IEDB_114703,IEDB_161730

• Article ID: 8717
  Silva R, Aguiar TQ, Oliveira C, Domingues L "Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii" - New Biotechnology 50 (2019) 1-8
  

  The blockage of the de novo pyrimidine biosynthetic pathway at the orotidine-5'-phosphate decarboxylase level was previously demonstrated to affect riboflavin production in the industrial producer fungus Ashbya gossypii. However, the molecular basis for the unusual sensitivity to uracil displayed by the pyrimidine auxotroph A. gossypii Agura3 was unknown. Here, uridine was shown to be the only intermediate of the pyrimidine salvage pathway able to fully restore this mutant's growth. Conversely, uracil, which is routinely used to rescue pyrimidine auxotrophs, had a dose-dependent growth-inhibitory effect. Uracil phosphoribosyltransferase (UPRT) is the pyrimidine salvage pathway enzyme responsible for converting uracil to uridine monophosphate in the presence of phosphoribosyl pyrophosphate (PRPP). Characterization of the A. gossypii UPRT, as produced and purified from Escherichia coli, revealed that uracil concentrations above 1 mM negatively affected its activity, thus explaining the hypersensitivity of the Agura3 mutant to uracil. Accordingly, overexpression of the AgUPRT encoding-gene in A. gossypii Agura3 led to similar growth on rich medium containing 5 mM uracil or uridine. Decreased UPRT activity ultimately favors the preservation of PRPP, which otherwise may be directed to other pathways. In A. gossypii, increased PRPP availability promotes overproduction of riboflavin. Thus, this UPRT modulation mechanism reveals a putative means of saving precursors essential for riboflavin overproduction by this fungus. A similar uracil-mediated regulation mechanism of the UPRT activity is reported only in two protozoan parasites, whose survival depends on the availability of PRPP. Physiological evidence here discussed indicate that it may be extended to other distantly related flavinogenic fungi.

  Ashbya gossypii, auxotrophy, phosphoribosyltransferase, pyrimidine metabolism, riboflavin production, uracil, uracil/uridine

  NCBI PubMed ID: 30590201
  Publication DOI: 10.1016/j.nbt.2018.12.004
  Journal NLM ID: 101465345
  Publisher: Amsterdam: Elsevier
  Correspondence: Domingues L
  Institutions: CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal
  
   
  
  Ashbya gossypii ATCC 10895
  CSDB ID 49669 (all data & tools)

Show legend Show as text

Structure type: monomer ; 377.1459 [M+H]+
C₁₇H₂₀O₆N₄
Trivial name: riboflavin, vitamin B2
Compound class: glycoside
Contained glycoepitopes: IEDB_114703,IEDB_161730

• Article ID: 8724
  Wang Z, Liu J, Zhong X, Li J, Wang X, Ji L, Shang X "Rapid characterization of chemical components in edible mushroom Sparassis crispa by UPLC-Orbitrap MS analysis and potential inhibitory effects on allergic rhinitis" - Molecules 24(16) (2019) ID 3014
  

  Sparassis crispa is a kind of edible fungus widely grows in the north temperate zone, which shows various medicinal properties. Due to the complexity of chemical constitutes of this species, few investigations have acquired a comprehensive configuration for the chemical profile of it. In this study, a strategy based on ultra-high performance liquid chromatography (UPLC) combined with Orbitrap mass spectrometer (MS) was established for rapidly characterizing various chemical components in S. crispa. Through the summarized MS/MS fragmentation patterns of reference compounds and systematic identification strategy, a total of 110 components attributed to six categories were identified for the first time. Moreover, allergic rhinitis (AR) is a worldwide inflammatory disease seriously affecting human health, and the development of drugs to treat AR has been a topic of interest. It has been reported that the extracts of S. crispa showed obvious inhibitory effects on degranulation of mast cell- and allergen-induced IgE and proinflammatory mediators, but the active components and specific mechanism were still not clear. Src family kinases (SFKs) participate in the initial stage of allergy occurrence, which are considered the targets of AR treatment. Herein, on the basis of that self-built chemical database, virtual screening was applied to predict the potential SFKs inhibitors in S. crispa, using known crystal structures of Hck, Lyn, Fyn, and Syk as receptors, followed by the anti-inflammatory activity evaluation for screened hits by intracellular calcium mobilization assay. As results, sparoside A was directly confirmed to have strong anti-inflammatory activity with an IC50 value of 5.06 ± 0.60 μM. This study provides a useful elucidation for the chemical composition of S. crispa, and demonstrated its potential inhibitory effects on AR, which could promote the research and development of effective agents from natural resources.

  identification, Sparassis crispa, anti-inflammatory activity, UPLC-MS, allergic rhinitis (AR)

  NCBI PubMed ID: 31434231
  Publication DOI: 10.3390/molecules24163014
  Journal NLM ID: 100964009
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Wang Z ; Shang X
  Institutions: Beijing Key Laboratory of Bioactive Substance and Functional Food, Beijing Union University, Beijing, China, College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong, China
  Methods: molecular modeling, extraction, biological assay, UHPLC-MS/MS, TIC, UHPLC-DAD
  
   
  
  Sparassis crispa
  CSDB ID 49697 (all data & tools)