Cell wall mannan of the pathogenic yeast Candida krusei was prepared using the antibiotic Benanomicin A, which has a lectin-like function. The chemical structure of this molecule was found to be similar to that of mannan prepared from the same yeast by the conventional method using Fehling reagent. Only a few degradation products were detected when the mannan prepared using Fehling reagent was subjected to alkali treatment (β-elimination), but multiple α-1,2-linked oligosaccharides were detected when the mannan purified with Benanomicin A was treated with alkali. These results indicate that most of the O-linked sugar chains in mannan were lost under conventional conditions when exposed to the strongly alkaline Fehling reagent. In contrast, the O-glycosidic bond in mannan was not cleaved and the O-linked sugar chains were maintained and almost intact following treatment with the mild novel preparation method using Benanomicin A. Therefore, we argue that the new mannan preparation method using Benanomicin A is superior to conventional methods. In addition, our study suggests that some yeast mannans, whose overall structure has already been reported, may contain more O-linked sugar chains than previously recognized.
β-elimination, antibiotic, Cell wall mannan, Benanomicin A, Candida krusei, O-linked sugar
NCBI PubMed ID: 30652080Publication DOI: 10.1002/2211-5463.12558Journal NLM ID: 101580716Publisher: West Sussex: John Wiley & Sons Ltd.
Correspondence: h-kobaya@niu.ac.jp
Institutions: Laboratory of Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Japan, Sasebo City General Hospital, Sasebo, Japan
Methods: 1H NMR, alkaline hydrolysis, extraction, cell growth, dialysis, precipitation, phenol-sulfuric acid assay, centrifugation, Folin phenol reagent method, Fehling treatment, phosphate measurement
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