Secondary metabolites of microorganisms have proven to be an excellent source of drugs. We isolated a new antibiotic, named pestiocandin (1), from a culture broth of a filamentous fungus, Pestalotiopsis humus FKI-7473, using a multidrug-sensitive budding yeast, S. cerevisiae 12geneΔ0HSR-iERG6. The structure of 1 was elucidated by various NMR studies. All geometric isomerisms of 1 were shown to be the E-form and two pyranose units of 1 were found to be glucose and galactose types. Compound 1 showed weak growth inhibition against Gram-positive and Gram-negative bacteria, yeasts and a filamentous fungus. It displayed more potent growth inhibition against multidrug-sensitive yeasts than wild-type yeasts.
construction, fermentation, Family, antifungal antibiotics, multidrug-sensitive yeast, fusarium-sambucinum
NCBI PubMed ID: 30232378Publication DOI: 10.1038/s41429-018-0102-7Journal NLM ID: 0151115Publisher: London: Nature Publishing Group
Correspondence: Omura S
; Shiomi K
Institutions: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan, Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan, Graduate School of Life and Environmental Sciences, Universitynof Tsukuba,Ibaraki, Japan
Methods: 13C NMR, 1H NMR, IR, HPLC, UV, extraction, ROESY, CC, HR-ESI-MS, HMBC, antimicrobial assay, centrifugation, COSY, antifungal activity test, HOHAHA, HSQC
Found