Glycosylation is an efficient strategy to modulate the solubility, stability, bioavailability and bioactivity of drug-like natural products. Biological methods, such as whole-cell biocatalyst, promise a simple but highly effective approach to glycosylate biologically active small molecules with remarkable regio- and stereo-selectivity. Herein, we use the entomopathogenic filamentous fungus Isaria fumosorosea ACCC 37814 to biotransform a panel of phenolic natural products, including flavonoids and anthraquinone, into their glycosides. Six new flavonoid (4-O-methyl)glucopyranosides are obtained and structurally characterized using high resolution mass and nuclear magnetic resonance spectroscopic techniques. These compounds further expand the structural diversity of flavonoid glycosides and may be used in biological study.
microbial transformation, flavonoids, Isaria fumosorosea, methylglycosylation
NCBI PubMed ID: 30875913Publication DOI: 10.3390/molecules24061028Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: Dou F
; Wang Z ; Li G ; Dun B
Institutions: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Methods: 13C NMR, 1H NMR, NMR-2D, HPLC, biosynthetic methods, extraction, LC-MS, cell growth, evaporation, HPLC-HR-ESI-MS
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