A novel polysaccharide AAMP-A70 (5.6 kDa) has been purified from the fruiting bodies of Amillariella mellea. Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-D-(1→6) linked Glcp residues substituted at O-3 by β-Glcp or α-D-(1→6)-linked Galp side chains. AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β. Mechanistically, AAMP-A70 promotes degradation of I kappa B-alpha and nuclear translocation of the NF-kappa B p65 subunit, and enhances phosphorylation of MAPKs. In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production. Our data demonstrate that AAMP-A70 activates macrophages via NF-kappa B/MAPK signaling pathways and the TLR2 receptor. Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
polysaccharides, oxidative stress, Morchella conica
NCBI PubMed ID: 28946308Publication DOI: 10.1016/j.foodchem.2017.07.154Journal NLM ID: 7702639Publisher: Elsevier Applied Science Publishers
Correspondence: Cheng HR
; Zhou YF
Institutions: Jilin Province Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China
Methods: 13C NMR, 1H NMR, SDS-PAGE, ELISA, HPLC, GPC, extraction, statistical analysis, DEAE, methylation analysis, immunofluorescence analyses, determination of NO production, cell viability assay, precipitation, phenol-sulfuric acid assay, centrifugation, FT-IR, TFA hydrolysis, western blotting, citokine production, MTT assay
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