Two new arborinane-type triterpenes, myrotheols A (1) and B (2), two new arborinane-type glycosides, myrothesides C (3) and D (4), together with four known diterpenes (5-8) were isolated from the ethyl acetate extract of the endolichenic fungus Myrothecium inundatum. The structures of new compounds 1-4 were elucidated by NMR and MS analyses. The absolute configuration of 1 was assigned by a single-crystal X-ray diffraction experiment. Compounds 3 and 4 represent the first two natural 4-O-methyl-α-D-mannosides. Compounds 1-8 exhibited cytotoxicity against K562 and RKO human cancer cell lines.
cytotoxicity, triterpene, endolichenic fungi, Myrothecium inundatum
NCBI PubMed ID: 30780164Publication DOI: 10.1055/a-0855-4051Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: Liu H
Institutions: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, International College, University of Chinese Academy of Sciences, Beijing, China, Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China, Institute of Applied Chemistry, Chemistry Department of L.N. Gumilyov Eurasian National University, Astana, Kazakhstan, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China
Methods: 13C NMR, 1H NMR, NMR-2D, IR, X-ray, UV, extraction, optical rotation measurement, CD, CC, RP-HPLC, cell growth, HR-ESI-MS, cytotoxicity assay, evaporation, sonication
Found