The isolations and purifications of secondary metabolites of marine-derived Penicillium sp. Hl were performed by means of column chromatography over silica gel and their structures were elucidated through the spectroscopic analysis of MS, NMR, and specific rotations. The bioactivities were assayed by paper diffusion. From the fermentation broth of marine-derived Penicillium sp. Hl, five compounds were isolated and identified as xylarinonericin E (1), fumitremorgin C (2), verruculogen (3), penicillide (4), and pyripyropene A (5), respectively. New compound 1 showed moderate antifungal activity against Fusarium oxysporum f. sp. cubense with MIC value of 32.0 μmol/mL. Compound 2 displayed antibacterial activity against Staphylococcus aureus with MIC value of 64.0 μglmL. Compounds 1 and 2 exhibit moderate antimicrobial activities.
antimicrobial activity, secondary metabolite, marine-derived Penicillium sp. Hl, xylarinonericin E, fumitremorgin C, pyripyropene A
Publication DOI: 10.7501/j.issn.0253-2670.2019.11.002Journal NLM ID: 9421493Publisher: Shao-Yang, China: Chung Tsao Yao Tsa Chih Pien Chi Pu
Correspondence: Wang C
Institutions: Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning, China, School of Pharmaceutical Sciences/Institutes of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, China
Methods: 13C NMR, 1H NMR, NMR-2D, DNA techniques, TLC, ESI-MS, UV, extraction, optical rotation measurement, cell growth, HR-ESI-MS, DEPT, antimicrobial assay, BLAST
Found