A rare fungal hopane-type triterpenoid glycoside fumihopaside A was identified by genome mining in combination with HPLC-MS/MS in Aspergillus fumigatus. Combining genetic deletions in A. fumigatus with heterologous reconstitutions in Aspergillus nidulans of the afum gene cluster, we identified one fungal squalene hopane cyclase AfumA charging the cyclization of the hopene skeleton, one cytochrome P450, and one UDP-glycosyltransferase. Bioassays indicated that fumihopaside A plays important roles in protecting A. fumigatus against heat or ultraviolet stress.
Aspergillus fumigatus, triterpenoid glycoside, fumihopaside A
NCBI PubMed ID: 30977375Publication DOI: 10.1021/acs.orglett.9b00984Journal NLM ID: 100890393Publisher: American Chemical Society
Correspondence: Liu H
; Yin WB
Institutions: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China, Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, USA, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China
Methods: 13C NMR, 1H NMR, NMR-2D, DNA techniques, ESI-MS, ESI-MS/MS, gene expression, HPLC-ELSD, HPLC-MS/MS
Found