Ten new diterpene glycosides virescenosides Z9-Z18 (1-10) together with three known analogues (11-13) and aglycon of virescenoside A (14) were isolated from the marine-derived fungus Acremonium striatisporum KMM 4401. These compounds were obtained by cultivating fungus on wort agar medium with the addition of potassium bromide. Structures of the isolated metabolites were established based on spectroscopic methods. The effects of some isolated glycosides and aglycons 15-18 on urease activity and regulation of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) production in macrophages stimulated with lipopolysaccharide (LPC) were evaluated.
secondary metabolites, Acremonium striatisporum, Diterpene glycosides, Marine fungi, urease activity
NCBI PubMed ID: 31671910Publication DOI: 10.3390/md17110616Journal NLM ID: 101213729Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: Zhuravleva OI
; Antonov AS ; Oleinikova GK ; Khudyakova YV <161070@rambler.ru>; Popov RS ; Denisenko VA ; Pislyagin EA ; Chingizova EA ; Afiyatullov SS
Institutions: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia, School of Natural Science, Far Eastern Federal University, Vladivostok, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, inhibition studies, TLC, biological assays, UV, extraction, gel chromatography, optical rotation measurement, CID-MS, CC, RP-HPLC, cell growth, HR-ESI-MS, determination of NO production, macrophage activity assay, evaporation
Found