Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Neisseria meningitidis [ICD11:
XN1DV 
]
NCBI PubMed ID: 37866526Publication DOI: 10.1016/j.ab.2023.115363Journal NLM ID: 0370535Publisher: Academic Press
Correspondence: A.D. Mallya <asha.mallya
seruminstitute.com>
Institutions: Department of Chemistry, University of Cape Town, Rondebosch, 7701, South Africa, Serum Institute of India Pvt Ltd, Serum Biopharma Park, 212/2, Hadapsar, Pune, 411 028, Maharashtra, India, National Institute for Biological Standards and Control, Medicines and Healthcare Products Regulatory Agency, South Mimms, AL3 6DP, United Kingdom
A selective and sensitive method was evaluated for quantitation of meningococcal X (Men X) polysaccharide in pentavalent meningococcal A, C, W, Y and X conjugate vaccine using different acid hydrolysis conditions like HCl, TFA, HF, HF-TFA, and HF-HCl. High-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using CarboPac PA10 column was used to identify the hydrolyzed products based on retention time and its comparison with monosaccharide standards. Complete release of glucosamine (GlcN) from Men X in monovalent bulk and pentavalent vaccine samples was achieved using HF hydrolysis at 80 °C for 2 h. The Men X HF-hydrolyzed polysaccharide to glucosamine along with the reference standard was identified using collision-induced dissociation (CID) electrospray mass spectroscopy and the MS/MS fragments of m/z 162, m/z 144 and m/z 84. Meningococcal polysaccharide concentration was determined with a correlation coefficient r2 >0.99 using polysaccharide reference standard. The serogroups A, W, and Y were converted to their monosaccharides units and quantified using this method however, milder acid hydrolysis 0.1 M HCl 80 °C 2 h for release of sialic acid for Men C polysaccharide was found to be more suitable. These methods will provide necessary tools and prove to be beneficial to laboratories developing new saccharide-based vaccine combinations.
HPAEC-PAD, acid hydrolysis, C, carbohydrate-based vaccine, electrospray mass spectroscopy, Meningococcal A, W, Y, and X polysaccharides
Structure type: homopolymer
Location inside paper: p. 115363-4, Men X
Compound class: O-polysaccharide, K-antigen, O-antigen, CPS
Contained glycoepitopes: IEDB_141807,IEDB_150077,IEDB_151531,IEDB_1711617
Methods: acid hydrolysis, HPAEC-PAD, HPAEC-PAD/ESI-MS
NCBI Taxonomy refs (TaxIDs): 487Reference(s) to other database(s): GTC:G19079RT, GlycomeDB:
34729
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error