Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
NCBI PubMed ID: 37178878Publication DOI: 10.1016/j.ijbiomac.2023.124837Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: G. Gojgic-Cvijovic <ggojgic
chem.bg.ac.rs>
Institutions: University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoseva 12, P.O. Box 473, 11000 Belgrade, Serbia, University of Belgrade, Vinca Institute of Nuclear Sciences, P.O. Box 522, 11001 Belgrade, Serbia, University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia, University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
In this study, levan from Bacillus licheniformis NS032 was modified in an aqueous medium by octenyl succinic anhydride (OSA), and the properties of the obtained derivatives were studied. The maximum efficiency in the synthesis reaction was achieved at 40 °C and a polysaccharide slurry concentration of 30 %. Increasing the reagent concentration (2-10 %) led to an increase in the degree of substitution (0.016-0.048). Structures of derivatives were confirmed by FTIR and NMR. Scanning electronic microscopy, thermogravimetry, and dynamic light scattering analyses showed that the derivatives with degrees of substitution of 0.025 and 0.036 retained levan's porous structure and thermostability and showed better colloidal stability than the native polysaccharide. The intrinsic viscosity of derivatives increased upon modification, while the surface tension of the 1 % solution was lowered to 61 mN/m. Oil-in-water emulsions prepared with sunflower oil (10 % and 20 %) by mechanical homogenization and 2 and 10 % derivatives in the continuous phase showed mean oil droplet sizes of 106-195 ?m, while the distribution curves exhibited bimodal character. The studied derivatives have a good capacity to stabilize emulsions, as they have a creaming index ranging from 73 % to 94 %. The OSA-modified levans could have potential applications in new formulations of emulsion-based systems.
Emulsification, levan, octenyl succinic anhydride
Structure type: structural motif or average structure
Location inside paper: p. 124837-2, Fig. 2
Trivial name: levan
Compound class: EPS, polysaccharide, fructan
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, NMR-2D, FTIR, viscosity measurement, statistical analysis, fermentation, dialysis, SEM, TGA, DLS, emulsion preparation
NCBI Taxonomy refs (TaxIDs): 1402Reference(s) to other database(s): GTC:G59138IY, GenDB:JF826527
Show glycosyltransferases
There is only one chemically distinct structure:
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