Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperNCBI PubMed ID: 37331538Publication DOI: 10.1016/j.ijbiomac.2023.125452Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: M.N. Bao <mtbao
ouc.edu.cn>
Institutions: Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China, College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266100, China
Polysaccharides derived from microorganisms exhibit diverse structures and bioactivities, making them promising candidates for the treatment of various diseases. However, marine-derived polysaccharides and their activities are relatively little known. In this work, fifteen marine strains were isolated from surface sediments in the Northwest Pacific Ocean for screening of EPS production. Planococcus rifietoensis AP-5 produced a maximum yield of EPS at 4.80 g/L. The purified EPS (referred to as PPS) had a molecular weight of 51,062 Da and contained amino, hydroxyl, and carbonyl groups as its major functional groups. PPS primarily consisted of →3)-α-D-Galp-(1→4)-α-D-Manp-(1→2)-α-D-Manp-(1→4)-α-D-Manp-(1→4,6)-α-D-Glcp-(1→6)-β-D-Galp-(1→, with a branch consisting of T-β-D-Glcp-(1→. Additionally, surface morphology of PPS was hollow, porous, and sphere-like stack. PPS primarily contained C, N, and O elements, with a surface area of 33.76 m2/g, a pore volume of 0.13 cc/g, and a pore diameter of 1.69 nm, respectively. Based on the TG curve, the degradation temperature of PPS was measured to be 247 °C. Furthermore, PPS demonstrated immunomodulatory activity through dose-dependently upregulating the expression level of cytokines. It significantly enhanced the cytokine secretion at a concentration of 5 μg/mL. To sum up, this study offers valuable insights for screening marine polysaccharide-based immunomodulators.
structure, exopolysaccharide, deep-sea bacteria, immunomodulatory potential, Planococcus sp.
Structure type: structural motif or average structure
Location inside paper: Fig. 4g, p. 125452-8
Compound class: EPS
Methods: 13C NMR, 1H NMR, methylation, GC-MS, sugar analysis, ELISA, FTIR, extraction, statistical analysis, cytokine production, SEM, TEM, AFM, DEPT-135, BLAST, UV-VIS, immunomodulatory activity analysis, XPS, BET analysis
NCBI Taxonomy refs (TaxIDs): 200991
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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