Aspergillus burnettii is a new species belonging to the A. alliaceus clade in Aspergillus subgenus Circumdati section Flavi isolated from peanut-growing properties in southern Queensland, Australia. A. burnettii is a fast-growing, floccose fungus with distinctive brown conidia and is a talented producer of biomass-degrading enzymes and secondary metabolites. Chemical profiling of A. burnettii revealed the metabolites ochratoxin A, kotanins, isokotanins, asperlicin E, anominine and paspalinine, which are common to subgenus Circumdati, together with burnettiene A, burnettramic acids, burnettides, and high levels of 14α-hydroxypaspalinine and hirsutide. The genome of A. burnettii was sequenced and an annotated draft genome is presented. A. burnettii is rich in secondary metabolite biosynthetic gene clusters, containing 51 polyketide synthases, 28 non-ribosomal peptide synthetases and 19 genes related to terpene biosynthesis. Functional annotation of digestive enzymes of A. burnettii and A. alliaceus revealed overlapping carbon utilisation profiles, consistent with a close phylogenetic relationship.
chemotaxonomy, Aspergillus, carbohydrate active enzymes, secondary metabolites, biosynthetic gene cluster analysis, molecular phylogeny
NCBI PubMed ID: 32702474Publication DOI: 10.1016/j.fgb.2020.103435Journal NLM ID: 9607601Publisher: Orlando, FL : Academic Press / Elsevier
Correspondence: Chooi YH
; Piggott AM
Institutions: School of Molecular Sciences, University of Western Australia, Crawley, Australia, Microbial Screening Technologies, Smithfield, Australia, Center for Bioprocess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark, BioEconomy, Research & Advisory, Copenhagen, Denmark, Department of Molecular Sciences, Macquarie University, Sydney, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, ESI-MS, biological assays, HPLC, optical rotation measurement, cell growth, HR-ESI-MS, enzymatic assay, antibacterial assay, DNA extraction, spectrophotometry, antitumor activity assay, centrifugation, antifungal activity test, amino acid sequence analysis, BLAST
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