The burnettramic acids are a new class of antibiotics from an Australian fungus Aspergillus burnettii. The rare bolaamphiphilic scaffold consists of β-D-mannose linked to a pyrrolizidinedione unit via a 26-carbon chain. The most abundant metabolite displayed potent in vitro antifungal activity. Comparative genomics identified the hybrid PKS-NRPS bua gene cluster, which was verified by heterologous pathway reconstitution in Aspergillus nidulans.
secondary metabolites, Aspergillus burnettii, pyrrolizidinediones
NCBI PubMed ID: 30735051Publication DOI: 10.1021/acs.orglett.8b04042Journal NLM ID: 100890393Publisher: American Chemical Society
Correspondence: Chooi YH
; Piggott AM
Institutions: Department of Molecular Sciences, Macquarie University, Sydney, Australia, School of Molecular Sciences, The University of Western Australia, Perth, Australia, Microbial Screening Technologies Pty. Ltd., Smithfield, Australia, Commonwealth Scientific and Industrial Research Organisation, North Ryde, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, biological assays, UV, extraction, RP-HPLC, cell growth, HR-ESI-MS, antibacterial assay, cytotoxicity assay, gene expression, antifungal activity test, BLAST, LC-DAD-MS, antiparasitic assay
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