Two new xanthone derivatives, pestalotiones A (1) and B (2), one new diphenyl ketone riboside, pestalotione C (7), and one new diphenyl ether, pestalotione D (8), along with five known compounds isosulochrin dehydrate (3), 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (4), isosulochrin (5), chloroisosulochrin (6), and pestalotether D (9), were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). The structures of the new compounds were unambiguously deduced by HRESIMS and 1D/2D-NMR spectroscopic data. Compound 6 showed modest cytotoxicity against the HeLa cell line with an IC50 value of 35.2 μM. Compound 9 also showed cytotoxic to the HeLa and MCF-7 cell lines, with IC50 values of 60.8 and 22.6 μM, respectively. Additionally, compounds 1 and 2 exhibited antioxidant activity in scavenging DPPH radical with IC50 values of 54.2 and 59.2 μg/mL, respectively.
structure elucidation, cytotoxicity, Antioxidant, secondary metabolites, Pestalotiopsis theae
NCBI PubMed ID: 31979166Publication DOI: 10.3390/molecules25030470Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: Guo L
; Liu S ; Lin J ; Niu S ; Liu L
Institutions: University of Chinese Academy of Sciences, Beijing, China, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, Jiangsu Key Laboratory for Biofunctional Molecules, College of Life Science and Chemistry, Jiangsu Second Normal University, Nanjing, China, School of Biological Medicine, Beijing City University, Beijing, China
Methods: 13C NMR, 1H NMR, NMR-2D, IR, ESI-MS, biological assays, UV, extraction, optical rotation measurement, CC, RP-HPLC, cell growth, HR-ESI-MS, antioxidant activities, evaporation, VLC, MTS assay
Found