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Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)

The structure was elucidated in this paper
NCBI PubMed ID: 36819025
Publication DOI: 10.3389/fmicb.2023.1108120
Journal NLM ID: 101548977
Publisher: Lausanne: Frontiers Research Foundation
Correspondence: W. Liu <liuwzh

sxnu.edu.cn>; B. Wu <wubaomei

sxnu.edu.cn>
Institutions: School of Life Sciences, Shanxi Normal University, Taiyuan, China, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China

Exopolysaccharides (EPSs) produced by lactic acid bacteria possess various bioactivities and potential attractions for scientific exploration and commercial development. An EPS-producing bacterial strain, RSG7, was previously isolated from the pepino and identified as Leuconostoc mesenteroides. Based on the analyses of high-performance size exclusion chromatography, high-performance ion chromatography, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and methylation, the RSG7 EPS was identified as a dextran with a molecular weight of 5.47 × 106 Da and consisted of α-(1→6) glycosidic linkages as backbone and α-(1→2), α-(1→3), α-(1→4), and α-(1→6) glycosidic linkages as side chains. Scanning electron microscopy observed a honeycomb-like porous structure of RSG7 dextran, and this dextran formed aggregations with irregular hill-shaped lumps according to atomic force microscopy analysis. Physical-chemical investigations suggested that RSG7 dextran possessed excellent viscosity at high concentration, low temperature, and high pH; showed a superior emulsifying capacity of tested vegetable oils than that of hydrocarbons; and owned the maximal flocculating activity (10.74 ± 0.23) and flocculating rate (93.46 ± 0.07%) in the suspended solid of activated carbon. In addition, the dextran could coagulate sucrose-supplemented milk and implied potential probiotics in vitro. Together, these results collectively describe a valuable dextran with unique characteristics for exploitation in food applications

Dextran, Structural characterization, Leuconostoc mesenteroides RSG7, pepino, physical-chemical properties

Structure type: structural motif or average structure ; 5470000
Location inside paper: table 1, Fig. 5
Compound class: EPS
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_140628,IEDB_140629,IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_153543,IEDB_153755,IEDB_158538,IEDB_158555,IEDB_232584,IEDB_2346541,IEDB_241101,IEDB_423115,IEDB_983931,SB_192

Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, GC-MS, sugar analysis, acid hydrolysis, FTIR, extraction, statistical analysis, rheological study, SEM, DEPT, AFM, emulsifying activity determination, HPIC, flocculation activity

NCBI Taxonomy refs (TaxIDs): 1245
Show glycosyltransferases

There is only one chemically distinct structure:

*OC[C@H]1OC(OC[C@H]2OC(OC[C@H]3OC(OC[C@H]4OC(OC[C@H]5OC(OC[C@H]6OC(OC[C@H]7OC(OC[C@H]8OC(OC[C@H]9OC(OC[C@H]%10OC(OC[C@H]%11OC(OC[C@H]%12OC(OC[C@H]%13OC(OC[C@H]%14OC(OC[C@H]%15OC(OC[C@H]%16OC(OC[C@H]%17OC(OC[C@H]%18OC(OC[C@H]%19OC(OC[C@H]%20OC(OC[C@H]%21OC(OC[C@H]%22OC(OC[C@H]%23OC(*)[C@H](O)[C@@H](O)[C@@H]%23O)[C@H](O)[C@@H](OC%23O[C@H](COC%24O[C@H](CO)[C@@H](O)[C@H](OC%25O[C@H](CO)[C@@H](O)[C@H](OC%26O[C@H](CO)[C@@H](O)[C@H](OC%27O[C@H](CO)[C@@H](OC%28O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%28O)[C@H](O)[C@H]%27O)[C@H]%26O)[C@H]%25O)[C@H]%24O)[C@@H](O)[C@H](O)[C@H]%23OC%23O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%23O)[C@@H]%22O)[C@H](O)[C@@H](OC%22O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%22O)[C@@H]%21O)[C@H](O)[C@@H](O)[C@@H]%20O)[C@H](O)[C@@H](O)[C@@H]%19O)[C@H](O)[C@@H](O)[C@@H]%18O)[C@H](O)[C@@H](OC%18O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%18O)[C@@H]%17O)[C@H](O)[C@@H](O)[C@@H]%16O)[C@H](O)[C@@H](O)[C@@H]%15O)[C@H](O)[C@@H](O)[C@@H]%14O)[C@H](O)[C@@H](OC%14O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%14O)[C@@H]%13O)[C@H](O)[C@@H](O)[C@@H]%12O)[C@H](O)[C@@H](O)[C@@H]%11O)[C@H](O)[C@@H](O)[C@@H]%10O)[C@H](O)[C@@H](OC%10O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%10O)[C@@H]9O)[C@H](O)[C@@H](O)[C@@H]8O)[C@H](O)[C@@H](O)[C@@H]7O)[C@H](O)[C@@H](O)[C@@H]6O)[C@H](O)[C@@H](OC6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H]5O)[C@H](O)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at ?DGlcp: large unit count (18) was reduced to 5