CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)

The structure was elucidated in this paper
NCBI PubMed ID: 38144786
Publication DOI: 10.1016/j.fochx.2023.100955
Journal NLM ID: 101751436
Publisher: Elsevier
Correspondence: L. Pan <panlei

fafu.edu.cn>
Institutions: Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, Fujian 350002, China, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Exopolysaccharides produced by Weissella cibaria has attracted increasing attention owing to their biological activity. Here, a strain was isolated from the home-made fermented octopus, which was identified as W. cibaria FAFU821. In addition, the polysaccharide were isolated and purified by cellulose DE-52 column and Sephadex G-100 column, and named EPS821-1. In this work, the structure of EPS821-1 and its cryoprotective activity on Bifidobacterium longum subsp. longum F2 were investigated in vitro. These results suggested that the EPS821-1 is a novel glucan, which mainly consists of α-(1→6) linkage with α-(1→4), α-(1→4,6) and α-(1→3,6) residue as branches. In addition, EPS821-1 existed the three-dimensional network structure and exhibited the excellent cryoprotective activities for B. longum subsp. longum F2, which was 2.75 folds higher than that of the controls. This study provided scientific evidence and insights for the application of EPS821-1 as cryoprotection in food field.

structure, exopolysaccharide, cryoprotective activity, Weissella cibaria FAFU821

Structure type: structural motif or average structure
Location inside paper: Fig. 3f
Compound class: EPS

Methods: 13C NMR, 1H NMR, methylation, GC-MS, FTIR, composition analysis, HPLC, statistical analysis, HPGPC, SEM, UV-vis, cryoprotective activity assay
Biological activity: B. longum subsp. longum F2 was protected by 0.9 % saline and 3 % (w/v) EPS821-1 with the survival rates of 16.47 %, 45.37 %, respectively.

NCBI Taxonomy refs (TaxIDs): 137591
Show glycosyltransferases

There is only one chemically distinct structure:

[*]OC[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@H](OC[C@H]4O[C@H](OC[C@H]5O[C@H](OC[C@H]6O[C@H](OC[C@H]7O[C@H](OC[C@H]8O[C@H]([*])[C@H](O)[C@@H](O)[C@@H]8O)[C@H](O)[C@@H](O)[C@@H]7O)[C@H](O)[C@@H](O)[C@@H]6O)[C@H](O)[C@@H](O[C@H]6O[C@H](CO[C@H]7O[C@H](CO[C@H]8O[C@H](CO)[C@@H](O)[C@H](O)[C@H]8O)[C@@H](O)[C@H](O[C@H]8O[C@H](CO[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@H]9O)[C@@H](O)[C@H](O[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@H]9O)[C@H]8O)[C@H]7O)[C@@H](O)[C@H](O[C@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@@H]5O)[C@H](O)[C@@H](O)[C@@H]4O)[C@H](O)[C@@H](O)[C@@H]3O[C@H]3O[C@H](CO[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H](O[C@H]4O[C@H](CO)[C@@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize