report error
Found 3 records.
Displayed records from 1 to 3
|
1. (CSDB ID: 22354) | report error |
| -4)-a-D-Galp3Ac-(1-2)-Gro-(3-P- | Show graphically |
|
Show legend Show as text |
Escherichia coli CFT073
(NCBI TaxID 199310,
species name lookup)
sanofi.com>; J.F.L. Duval <jerome.duvaluniv-lorraine.fr>; J.M. Chigo <jmghigopasteur.fr>Bacterial biofilms are surface-attached communities that are difficult to eradicate due to a high tolerance to antimicrobial agents. The use of non-biocidal surface-active compounds to prevent the initial adhesion and aggregation of bacterial pathogens is a promising alternative to antibiotic treatments and several antibiofilm compounds have been identified, including some capsular polysaccharides released by various bacteria. However, the lack of chemical and mechanistic understanding of the activity of these polymers limits their use to control biofilm formation. Here, we screen a collection of 31 purified capsular polysaccharides and first identify seven new compounds with non-biocidal activity against Escherichia coli and/or Staphylococcus aureus biofilms. We measure and theoretically interpret the electrophoretic mobility of a subset of 21 capsular polysaccharides under applied electric field conditions, and we show that active and inactive polysaccharide polymers display distinct electrokinetic properties and that all active macromolecules share high intrinsic viscosity features. Despite the lack of specific molecular motif associated with antibiofilm properties, the use of criteria including high density of electrostatic charges and permeability to fluid flow enables us to identify two additional capsular polysaccharides with broad-spectrum antibiofilm activity. Our study therefore provides insights into key biophysical properties discriminating active from inactive polysaccharides. The characterization of a distinct electrokinetic signature associated with antibiofilm activity opens new perspectives to identify or engineer non-biocidal surface-active macromolecules to control biofilm formation in medical and industrial settings.
Escherichia coli, capsular polysaccharide, Staphylococcus aureus, antibiofilm activity, bacterial biofilms
Structure type: polymer chemical repeating unit ; n=1500-2500|
2. (CSDB ID: 26415) | report error |
| /Variants 0/-+ | -6)-a-D-Glcp-(1-4)-a-Neup5Ac-(2- /Variants 0/ is: ?%Ac-9)- OR (exclusively) ?%Ac-7)- | Show graphically |
|
Show legend Show as text |
Neisseria meningitidis Y
(NCBI TaxID 648194,
species name lookup)
]sanofi.com>; J.F.L. Duval <jerome.duvaluniv-lorraine.fr>; J.M. Chigo <jmghigopasteur.fr>Bacterial biofilms are surface-attached communities that are difficult to eradicate due to a high tolerance to antimicrobial agents. The use of non-biocidal surface-active compounds to prevent the initial adhesion and aggregation of bacterial pathogens is a promising alternative to antibiotic treatments and several antibiofilm compounds have been identified, including some capsular polysaccharides released by various bacteria. However, the lack of chemical and mechanistic understanding of the activity of these polymers limits their use to control biofilm formation. Here, we screen a collection of 31 purified capsular polysaccharides and first identify seven new compounds with non-biocidal activity against Escherichia coli and/or Staphylococcus aureus biofilms. We measure and theoretically interpret the electrophoretic mobility of a subset of 21 capsular polysaccharides under applied electric field conditions, and we show that active and inactive polysaccharide polymers display distinct electrokinetic properties and that all active macromolecules share high intrinsic viscosity features. Despite the lack of specific molecular motif associated with antibiofilm properties, the use of criteria including high density of electrostatic charges and permeability to fluid flow enables us to identify two additional capsular polysaccharides with broad-spectrum antibiofilm activity. Our study therefore provides insights into key biophysical properties discriminating active from inactive polysaccharides. The characterization of a distinct electrokinetic signature associated with antibiofilm activity opens new perspectives to identify or engineer non-biocidal surface-active macromolecules to control biofilm formation in medical and industrial settings.
Escherichia coli, capsular polysaccharide, Staphylococcus aureus, antibiofilm activity, bacterial biofilms
Structure type: polymer chemical repeating unit|
3. (CSDB ID: 26416) | report error |
| /Variants 0/-+ | -6)-a-D-Galp-(1-4)-a-Neup5Ac-(2- /Variants 0/ is: ?%Ac-9)- OR (exclusively) ?%Ac-7)- | Show graphically |
|
Show legend Show as text |
Neisseria meningitidis W135
(Ancestor NCBI TaxID 487,
species name lookup)
]sanofi.com>; J.F.L. Duval <jerome.duvaluniv-lorraine.fr>; J.M. Chigo <jmghigopasteur.fr>Bacterial biofilms are surface-attached communities that are difficult to eradicate due to a high tolerance to antimicrobial agents. The use of non-biocidal surface-active compounds to prevent the initial adhesion and aggregation of bacterial pathogens is a promising alternative to antibiotic treatments and several antibiofilm compounds have been identified, including some capsular polysaccharides released by various bacteria. However, the lack of chemical and mechanistic understanding of the activity of these polymers limits their use to control biofilm formation. Here, we screen a collection of 31 purified capsular polysaccharides and first identify seven new compounds with non-biocidal activity against Escherichia coli and/or Staphylococcus aureus biofilms. We measure and theoretically interpret the electrophoretic mobility of a subset of 21 capsular polysaccharides under applied electric field conditions, and we show that active and inactive polysaccharide polymers display distinct electrokinetic properties and that all active macromolecules share high intrinsic viscosity features. Despite the lack of specific molecular motif associated with antibiofilm properties, the use of criteria including high density of electrostatic charges and permeability to fluid flow enables us to identify two additional capsular polysaccharides with broad-spectrum antibiofilm activity. Our study therefore provides insights into key biophysical properties discriminating active from inactive polysaccharides. The characterization of a distinct electrokinetic signature associated with antibiofilm activity opens new perspectives to identify or engineer non-biocidal surface-active macromolecules to control biofilm formation in medical and industrial settings.
Escherichia coli, capsular polysaccharide, Staphylococcus aureus, antibiofilm activity, bacterial biofilms
Structure type: polymer chemical repeating unit| New query | Export IDs | Home | Help |
Execution: <1 sec