Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11:
XN3PW 
]
NCBI PubMed ID: 37402017Publication DOI: 10.1007/s10719-023-10125-8Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: J.Z. Deng <james_deng
merck.com>
Institutions: Vaccine Analytical Research & Development, 770 Sumneytown Pike, P. O. Box 4, West Point, PA, WP46-3305, 19486, USA, Small Molecule Analytical Research & Development, Analytical Research & Development Merck & Co., Inc, Rahway, NJ, USA
Streptococcus pneumoniae is a highly invasive bacterial pathogen that can cause a range of illnesses. Pneumococcal capsular polysaccharides (CPS) are the main virulence factors that causes invasive pneumococcal disease (IPD). Pneumococcal CPS serotype 7F along with a few other serotypes is more invasive and likely to cause IPD. Therefore, 7F is a target for pneumococcal vaccine development, and is included in the two recently approved multi-valent pneumococcal conjugated vaccines, i.e. VAXNEUVANCE and PREVNAR 20.To support process and development of our 15-valent pneumococcal conjugated vaccine (PCV15), chromatographic methods have been developed for 7F polysaccharide and conjugate characterization. A size-exclusion chromatography (SEC) method with UV, light scattering and refractive index detections was employed for concentration, size and conformation analysis. A reversed-phase ultra-performance liquid chromatography (RP-UPLC) method was used for analysis of conjugate monosaccharide composition and degree of conjugation. The collective information obtained by these chromatographic analysis provided insights into the pneumococcal conjugate and conjugation process.
pneumococcal conjugate vaccine, Dynamic light scattering (DLS), Multi-angle light scattering (MALS), Pneumococcal capsular polysaccharide 7F, Size-exclusion chromatography (SEC), Ultra-performance liquid chromatography (UPLC)
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Trivial name: glycan epitope (glycotope)
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_136044,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 1H NMR, SEC, conjugation, MALS, DLS, RP-UPLC, SEC-UV-MALS-DLS-RI
NCBI Taxonomy refs (TaxIDs): 1313Reference(s) to other database(s): GTC:G24963GX, GlycomeDB:
16860
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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