2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics.
2, marine-derived fungi, 4-dichlorophenol, DCP metabolites, catechol dioxygenase, invertebrate symbionts
NCBI PubMed ID: 32392868Publication DOI: 10.3390/ijms21093317Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Nikolaivits E
; Agrafiotis A ; Baira E ; Tsafantakis N ; Fokialakis N ; Le Goff G ; Ouazzani J ; Chavanich SA ; Benayahu Y ; Topakas E
Institutions: Biochemical and Chemical Process Engineering, Division of Sustainable Process Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, Luleå, Sweden, Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece, Division of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece, Institut de Chimie des Substances Naturelles, ICSN, CNRS, Gif sur Yvette, France, Faculty of Science, Chulalongkorn University, Bangkok, Thailand, School of Zoology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Methods: PCR, DNA sequencing, HPLC, cell growth, enzymatic assay, DNA extraction, spectrophotometry, sonication, centrifugation, filtration, BLAST, UHPLC-ESI-HRMS
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