Athelia rolfsii TEMG (MH 236106) an exopolysaccharide (EPS) producing fungal strain was isolated and identified. Extraction, purification, characterization, antimicrobial, antioxidant, antiviral and antitumor activities of the polysaccharide were investigated. It characterized as a homopolysaccharide of glucose with a molecular weight of 345.622 kDa. The identification of the polysaccharide was conducted using scanning electron microscopy, energy dispersive X-ray analysis, 1H and 13C NMR spectra. The existence of β-1,3 and β-1,6 linkages suggests that EPS could be scleroglucan. The purified scleroglucan showed considerable antibacterial and antioxidant activities. The results indicated that, there was no cytotoxicity on normal cell (W138) and no effect on tumor cell lines including HepG2 and PC3 showing IC50 of 5096.83, 5885.80 and 4803.90 μg/mL, respectively. The results showed also that Sclg could reduce the cytopathic effect by 50% (EC50) at 15 and 50 μg/mL of herpes simplex virus type-1 (HSV-1) and influenza virus (H5N1), respectively.
scleroglucan, Athelia rolfsii TEMG, exopolysaccharide characterization, antiviral activity against H5N1 and HSV-1
NCBI PubMed ID: 32622769Publication DOI: 10.1016/j.ijbiomac.2020.06.272Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Farid MAM
Institutions: Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Div., National Research Centre, Cairo, Egypt, Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt, Plant Pathology Branch, Agricultural Botany Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
Methods: 13C NMR, 1H NMR, PCR, DNA sequencing, X-ray, acid hydrolysis, biological assays, HPLC, UV, viscosity measurement, cell growth, dialysis, antioxidant activities, cell viability assay, DNA extraction, rheological study, precipitation, spectrophotometry, SEM, antitumor activity assay, evaporation, antimicrobial assay, centrifugation, antiviral assay, BLAST
Found